The invention discloses a low temperature phase change cool storage agent and a preparation method thereof. The low temperature phase change cool storage agent comprises, by mass, 8% to 19.5% of ammonium chloride, 0.1% to 2% of dipotassium phosphate, 0.1% to 1% of sodium hydroxyethyl cellulose, 0.05% to 0.1% of nano-titanium dioxide and the balance deionized water. The preparation method includes adding the ammonium chloride, the dipotassium phosphate and the nano-titanium dioxide in the deionized water, uniformly mixing to obtain a solution, heating the solution to 70 DEG C to 80 DEG C, then slowly adding the sodium hydroxyethyl cellulose in the solution under high speed stirring, and uniformly stirring to obtain the low temperature phase change cool storage agent. Compared with low temperature phase change cool storage agents and preparation methods in prior art, the low temperature phase change cool storage agent and the preparation method thereof have the advantages that the low temperature phase change cool storage agent is low in phase transition temperature, free of salt crystal separation, high in phase change potential heat value, free of potential heat value decay after overturn circulation and the like, raw materials are easy to obtain, the production is easy, the low temperature phase change cool storage agent is safe and non-toxic, and the low temperature phase change cool storage agent has a potential application value in food fresh keeping and biological vaccine cold chain transportation.