The invention discloses a wood ganoderma lucidum nutrition wine and a preparation method thereof, and belongs to the compound wind production field. The wood ganoderma lucidum nutrition wine is characterized in that raw materials mainly comprise distilled white spirit, purified water, wood ganoderma lucidum powder and medicine and food dual-purpose items of kudzu vine roots, Chinese wolfberry fruits, liquorices and chrysanthemums. The preparation method includes subjecting the medicine and food dual-purpose items to washing and picking preprocessing and mixing and smashing the medicine and food dual-purpose items, brewing the smashed mixture with the purified water at the temperature of 80 DEG C to 100 DEG C, and performing stirring and dilution; adding the distilled white spirit and the wood ganoderma lucidum powder to perform mixing and submerging, subjecting the mixture to aging treatment, and performing ageing treatment, verse osmosis and leaching supernate; and preparing the wood ganoderma lucidum nutrition wine through mixing, blending, storage, clarifying, sterilization and packaging processes. The wood ganoderma lucidum nutrition wine is not only rich in ingredients beneficial to human health of ganoderan, ganoderma triterpenoids, various amino acid and vitamin and the like, but also keeps the white spirit taste, and has wood ganoderma lucidum aroma and medicine flavor of the medicine and food dual-purpose items. The preparation method is unique, the technological process is clean and environment-friendly, and the nutrition wine is applicable to mechanization production.