The invention discloses a release agent for battery grid casting and a preparation method thereof. The release agent comprises the following raw materials in parts by weight: 0.2-0.6 parts of polytetrafluoroethylene, 0.1-0.3 parts of aluminum dihydrogen phosphate, cork 1-5 parts of powder, 0.2-0.6 parts of sodium silicate, 0.6-1.5 parts of glass fiber powder, 0.1-0.6 parts of attapulgite, 0.6-2.5 parts of nano-silicon dioxide, 3-8 parts of modified aluminum tripolyphosphate, Adhesive 0.1-0.5 parts, stabilizer 0.2-0.8 parts, deionized water 90-120 parts. The preparation method adopts conventional mixing, heating and stirring. The mold release agent of the present invention has good sprayability, adhesion, temperature resistance, small surface tension, high compressive strength, smooth exhaust, can significantly reduce the phenomenon of air holes and cracks in the grid, and can also reduce the number of spray molds , improve production efficiency and save costs.