The invention discloses a preparation method and application of a dual-target acid-sensitive
Prussian blue drug-carrying
system, which can effectively solve the problems of
leukemia drug resistance and liver and
spleen infiltration. The technical solution is, firstly, the acid-sensitive
polyethylene glycol-polyethyleneimine
polymer is adsorbed on the surface of
Prussian blue nanoparticles through electrostatic and
hydrogen bonding; secondly, CXCR4 antagonistic polypeptide (E5) and
hyaluronic acid are chemically bonded to Polyethyleneimine molecules on the surface of
Prussian blue nanoparticles are connected; again,
daunorubicin is adsorbed on the surface of Prussian blue nanoparticles through
hydrogen bonding. The preparation method of the present invention is simple and mild, the material is safe and easy to obtain, and has good
biocompatibility. The prepared carrier has long-term
blood circulation ability, tumor
cell double-targeting ability,
lysosome escape ability, and acid-sensitive
drug release ability, and can effectively Enhance the
therapeutic effect of
leukemia in mice and inhibit the homing of
bone marrow and the infiltration of liver and
spleen.