The invention discloses a daunorubicin or daunorubicin hydrochloride liposome injection and a preparation process thereof. The injection is prepared from daunorubicin or daunorubicin hydrochloride, neutral phospholipids, negatively charged phospholipids, cholesterol, antioxidants, organic acids, alkalis, sugars, buffers, and water for injection. The preparation process includes preparing blank lipids Plastids, homogenized liposomes, encapsulating drugs, purifying and solidifying liposomes, constant volume, sterilization, subpackaging, and preservation. The injection has good stability, high encapsulation rate, low cost, less toxic and side effects, and the preparation process is simple and easy.