The invention relates to the fields of food and food processing and particularly relates to a children beefsteak capable of supplementing docosahexaenoic acid and a preparation method of the children beefsteak. The children beefsteak capable of supplementing the docosahexaenoic acid, provided by the invention, is prepared from raw materials, i.e., beef short loin, common salt, white granulated sugar, phosphate, isolated protein, starch, docosahexaenoic acid, spices, black pepper powder, monosodium glutamate, flavoring essence, glutamine transaminage and ice water through the processes of unfreezing, segmentation, feed solution preparing, injection, twice rolling and kneading, encapsulation, standing, quick freezing and the like. An unsaturated fatty acid, i.e., docosahexaenoic acid can be uniformly added into the children beefsteak through the method provided by the invention, the total intake amount of the docosahexaenoic acid of children is accurately calculated and controlled by the beefsteak intake of the children, and finally, the aim of reasonably supplementing the docosahexaenoic acid is achieved.