The invention provides a preparation method of 2, 3, 3, 3-tetrafloropropylene. The method comprises the following steps: with monochlorodifluoromethane and monochloro methane which are in a molar ratio of (0.5-4):1 as reaction raw materials, preheating the reaction raw materials, then mixing with superheated steam, wherein the volume ratio of the superheated steam to the reaction raw materials is (1-20):1, the temperature of the superheated steam is 900-1100 DEG C, the temperature in the reaction section is 600-1100 DEG C, and the stay time of reactants in the reaction section is 0.01-1s; and quenching, deacidifying, drying and rectifying and separating the product mixed flow to obtain 2, 3, 3, 3-tetrafloropropylene. The method provided by the invention overcomes the deficiency that a hollow tube is prone to carbonization in pyrolysis and side reactions are complex, the equipment maintenance rate is reduced, and meanwhile, the reaction period is prolonged, and the utilization ratio of the device is improved. The method provided by the invention has the characteristics of use of easily available raw materials, simple reaction process and the like. The obtained 2, 3, 3, 3-tetrafloropropylene has the advantages of zero ODP (Ozone Depletion Potential) and low GWP (Global Warming Potential), and is used as a substituent of automotive air conditionor refrigerant.