The invention discloses a pressure-reduced ozone-charged fruit and vegetable storage method. The storage method comprises the following steps: A, manufacturing a closed pressure-proof storeroom body; B, performing heat preservation in the storeroom; C, setting a full-automatic control system; D, storing fresh fruits and vegetables; E, storing the fresh fruits and vegetables under reduced pressure; F, charging the storeroom with ozone; G, humidifying the storehouse. The closed pressure-proof storeroom body is adopted, so that the pressure and the temperature can be rapidly reduced, the oxygen can be rapidly reduced, and the harmful gas components in the storeroom can be quickly removed, therefore the physiological health of the fruits and vegetables is guaranteed to the largest extent, the stored fruits and vegetables do not age, do not yellow, and do not lose weight, the different varieties of fruits and vegetables can be placed in a mixing manner, the storage amount is large, the operation is flexible, and the use is convenient. The method is adopted to build the fruit and vegetable reduced-pressure storeroom, the preservation cost can be reduced by 30%, the method is simple, the cost is low and the effect is remarkable; test proves that the storage method can remarkably prolong the storage period, the storage period of the vegetables can be 60 days or above, and the storage period of the fruits can be 90 days or above.