The invention discloses an automatic detection method for a large transfusion production line, including the following steps: 1, making a transfusion bottle filled with liquid medicine rotate in a high-speed, then rapidly stopping the transfusion bottle, so that the liquid medicine in the bottle has a self-rotating movement under the inertia; 2, picking-up a plurality of images of the transfusion bottle in which the liquid medicine is in a self-rotating state; 3, respectively digitally processing and mutually comparing to the plurality of images, distinguishing the exogenous impurity in the liquid medicine, in order to judge whether the bottle of liquid medicine is qualified. The invention also discloses an automatic detection apparatus for a large transfusion production line, which is characterized by including a rotation device, a controller, a video camera and an image processing apparatus, wherein, the output end of the controller is connected with the control terminal of the rotation device, a gripping device for fixing up the transfusion bottle, a rotating work station and a detecting work station are provided on the rotation device, and the video camera outputs data to the image processing apparatus. The invention can automatically detect whether impurity exists in the liquid medicine, with high testing accuracy and fast testing velocity.