The invention belongs to the field of design of imaging optical systems and discloses a wide-waveband and large-field-depth microscope objective optical system used for a flow cytometry. The wide-waveband and large-field-depth microscope objective optical system used for the flow cytometry aims at solving the problems that an existing microscope objective of the flow cytometry is narrow in waveband and small in field depth in the prior art. The wide-waveband and large-field-depth microscope objective optical system used for the flow cytometry comprises a first lens assembly, a second lens assembly, a cubic phase board, a third lens assembly, an eighth lens, a fourth lens assembly and a fifth lens assembly which are sequentially arranged along the optical axis from left to right; the fifth lens assembly is a plus lens assembly and comprises a binary diffraction optical element, the flow face, close to the flow cytometry, of the binary diffraction optical element is a binary optical diffraction face, the base face is a plane, the cubic phase board is located at the exit pupil position of the microscope objective optical system, and the face, deviating from a flow cytometry flow chamber, of the binary diffraction optical element is a cubic surface. After the binary diffraction optical element is introduced, chromatic aberration is well corrected. Meanwhile, after the cubic phase board is introduced, the field depth of the microscope objective is extended by twenty times to reach + / -16.8 micrometers.