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33 results about "Substrate channeling" patented technology

Substrate channeling is the passing of the intermediary metabolic product of one enzyme directly to another enzyme or active site without its release into solution. When several consecutive enzymes of a metabolic pathway channel substrates between themselves, this is called a metabolon. Channeling can make a metabolic pathway more rapid and efficient than it would be if the enzymes were randomly distributed in the cytosol, or prevent the release of unstable intermediates. It can also protect an intermediate from being consumed by competing reactions catalyzed by other enzymes.

Recombinant bacillus subtilis and construction method and application thereof

ActiveCN108998402AReduce metabolic burdenDoes not affect life activitiesBacteriaHydrolasesLife activityBacterial strain
The invention discloses a recombinant bacillus subtilis and a construction method and an application thereof, which belong to the technical field of genetic engineering. The method takes cell self functional membrane microdomain FMMs as a space frame, and constructs a multienzyme complex by using the specific marker proteins FloA and FloT, an artificial substrate channel is constructed, the metabolic burden of the cells can be effectively reduced, and the multienzyme complex is attached to plasmalemma , which facilitates the transport of a product from the intracellular to the extracellular. The recombinant bacillus subtilis constructed by the method efficiently synthesizes GlcNAc without affecting cell life activities, and a toxic intermediate metabolite GlcN-6-P can also be confined to the vicinity of the plasmalemma to reduce or eliminate the inhibition of cell viability. During a shake flask fermentation of a composite medium, the yield of acetylglucosamine in contrast bacterial strain BSGC is only 0.45 g / L, and the BSGAT acetylglucosamine output is increased to 5.29g / L, which is 11.76 times that of the contrast bacterial strain. The recombinant bacillus subtilis construction method of the invention is simple and convenient to use, and has the good application prospect.
Owner:JIANGNAN UNIV

Separation media, multiple electrospray nozzle system and method

A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles for the same fluid for analysis. This invention dramatically increases the sensitivity of microchip electrospray devices compared to prior disclosed systems and methods. The silicon chip having the packed microchannels disclosed herein finds application in coupling with mass spectrometry for sample analysis. Also disclosed is a separation block having multiple through-substrate channels filled with a separation material such as polymer monolith which can be stacked in multiple blocks for sequential two-dimensional chromatographic separation and integrated with the electrospray device.
Owner:ADVION

Split-gate SONOS (Semiconductor Oxide Nitride Oxide Semiconductor) memory device

The invention provides a split-gate SONOS (Semiconductor Oxide Nitride Oxide Semiconductor) memory device. The split-gate SONOS memory device comprises a gate oxide layer and a nitrogen oxide layer which are formed on a substrate, wherein a selection tube polysilicon gate, a top end oxide layer and an isolation layer are arranged on the gate oxide layer; a memory tube polysilicon gate is arrangedon the nitrogen oxide layer; the nitrogen oxide layer and one side of the memory tube polysilicon gate are close to the isolation layer; a memory tube P-type well is positioned below the selection tube polysilicon gate and the memory tube polysilicon gate; the substrate channel between the source end of the selection tube and the selection tube polysilicon gate is provided with a P-type boron injection region; and the channel of the P-type substrate below the memory tube polysilicon gate is provided with an N-type arsenic injection region. According to the invention, a single-side boron injection region of the selection tube and a source-end single-side arsenic or antimony injection region are formed, and boron in the boron injection region is diffused into a channel of the selection tubeto realize threshold voltage adjustment of the selection tube; an N-type arsenic injection region is formed through the threshold voltage adjustment of the memory tube; and finally, threshold voltageadjustment of the selection tube and threshold voltage adjustment of the memory tube device are not influenced mutually, the device performance is improved, and the manufacturing cost is reduced.
Owner:SHANGHAI HUAHONG GRACE SEMICON MFG CORP

Oven for baking battery substrate

The invention provides an oven for baking a battery substrate. An upper row roller set and a lower row of roller set which are distributed alternately are arranged inside an oven box; a substrate channel is formed in a vertical direction between the upper row of roller set and the lower row of roller set; a heating pipe and a substrate channel are alternately distributed; the substrate can move inside the heating channel while being heated; an air blowing mechanism which is arranged inside the box body timely takes away evaporated water vapor and organic solvent when the substrate is heated and vacuumization and inert gas injection are not required; an unreeling mechanism, the box body and a rolling-up mechanism are sequentially connected along the movement direction of the substrate. The oven for baking the battery substrate has the advantages that enabling unreeling, baking and rolling up to be achieved in one step and simplifying working processes; enabling the substrate to be unfolded completely inside the box body and being uniform in heating; shortening the baking time and improving the baking efficiency due to the fact that the air blowing mechanism timely takes away evaporated water vapor and organic solvent when the substrate is heated; heating double sides of the substrate and being applicable to the substrate with one coated side or two coated sides due to the fact that the heating pipe and the substrate are alternately arranged.
Owner:SHENZHEN HAONENG TECH
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