The invention discloses high-efficiency specimen corrosion preventing preserving fluid and a preparation method thereof. The preserving fluid is prepared from the raw materials in parts by weight: 10 to 60 parts of methyl alcohol, 0.01 to 10 parts of polyoxyethylene fatty alcohol ether, 1 to 18 parts of hexyl alcohol, 0.01 to 15 parts of benzalkonium bromide, 2 to 20 parts of salt, 0.05 to 12 parts of phenol, 0.5 to 20 parts of potassium nitrate, 5 to 25 parts of white alum, 0.05 to 15 parts of glycerinum, 0.1 to 15 parts of benzoic acid, 0.05 to 8 parts of isothiazolone, 0.1 to 10 parts of picric acid, 0.05 to 2 parts of penicillin and 0.05 to 2 parts of streptomycin. The preparation method comprises the steps of putting the polyoxyethylene fatty alcohol ether, the benzalkonium bromide, the salt, the phenol, the potassium nitrate, the white alum, the benzoic acid, the isothiazolone, the picric acid, the penicillin and the streptomycin into the methyl alcohol to be stirred, adding the hexyl alcohol and the glycerinum after the materials are complete dissolved and evenly stirring to obtain the high-efficiency specimen corrosion preventing preserving fluid. The high-efficiency specimen corrosion preventing preserving fluid disclosed by the invention avoids formaldehyde, has the advantages of slow volatilization, no pungent smell, high safety and good storage effect, cannot mildew after being stored for a long time, can enables specimens to absorb water to be moisturized, not to be prone to being dry dried and to keep a flexible state, can be repeatedly utilized for many times and is not prone to being damaged.