The invention provides a
nucleic acid isothermal amplification
reaction system, and a making method and applications thereof. The
nucleic acid isothermal amplification
reaction system comprises a capillary main body, at least one sample amplification reaction
liquid layer is filled in the capillary main body, and two ends of the sample amplification reaction
liquid layer are respectively sequentially filled with a gas layer and at least one
liquid layer; and the applications comprise an application of the
nucleic acid isothermal amplification
reaction system in nucleic acid amplification and an application of the nucleic acid isothermal amplification reaction
system in the preparation of
disease detection medicines. The
system eliminates a problem of the leakage
pollution of an
aerosol in a nucleic acid isothermal amplification reaction, and substantially improves the credibility of an isothermal amplification technology; and the
system has the advantages of extremely simple operation, low cost, easy integration, diversified heating mode and diversified
signal exciting mode, makes the whole flow completely divorced from an external power supply, and allows a final
signal to be directly observed by naked eyes.