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Nucleic acid isothermal amplification reaction system, and making method and applications thereof

The invention provides a nucleic acid isothermal amplification reaction system, and a making method and applications thereof. The nucleic acid isothermal amplification reaction system comprises a capillary main body, at least one sample amplification reaction liquid layer is filled in the capillary main body, and two ends of the sample amplification reaction liquid layer are respectively sequentially filled with a gas layer and at least one liquid layer; and the applications comprise an application of the nucleic acid isothermal amplification reaction system in nucleic acid amplification and an application of the nucleic acid isothermal amplification reaction system in the preparation of disease detection medicines. The system eliminates a problem of the leakage pollution of an aerosol in a nucleic acid isothermal amplification reaction, and substantially improves the credibility of an isothermal amplification technology; and the system has the advantages of extremely simple operation, low cost, easy integration, diversified heating mode and diversified signal exciting mode, makes the whole flow completely divorced from an external power supply, and allows a final signal to be directly observed by naked eyes.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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