The invention provides a 
nucleic acid isothermal amplification 
reaction system, and a making method and applications thereof. The 
nucleic acid isothermal amplification 
reaction system comprises a capillary main body, at least one sample amplification reaction 
liquid layer is filled in the capillary main body, and two ends of the sample amplification reaction 
liquid layer are respectively sequentially filled with a gas layer and at least one 
liquid layer; and the applications comprise an application of the 
nucleic acid isothermal amplification 
reaction system in nucleic acid amplification and an application of the nucleic acid isothermal amplification reaction 
system in the preparation of 
disease detection medicines. The 
system eliminates a problem of the leakage 
pollution of an 
aerosol in a nucleic acid isothermal amplification reaction, and substantially improves the credibility of an isothermal amplification technology; and the 
system has the advantages of extremely simple operation, low cost, easy integration, diversified heating mode and diversified 
signal exciting mode, makes the whole flow completely divorced from an external power supply, and allows a final 
signal to be directly observed by naked eyes.