A double frequency conversion efficiency testing arrangement of centrosymmetry material micro-nanometer structural elements is disclosed that comprises laser, collimated beam expanding system, Polaroid, incident light fence, focusing microscope, object supporter, turntable of horizontal angle, multi-dimension holder, double frequency filter disc, casing of barrier, laser powermeter and big bottom desk, the laser emitted by laser device goes through the collimated beam expanding system, Polaroid and incident light fence, and observed with focusing microscope, and adjusted with the turntable of horizontal angle and the multi-dimension holder, then emitted into the measured example, the double frequency light that its frequency is doubled in the measured example is received and measured by the laser powermeter to display the light intensity value of the double frequency light, the light intensity value is compared with the light intensity value that the measured example and the filter disc are not placed, the double frequency conversion efficiency can be gained. The position of the measured example is adjusted quickly by multi-dimension holder and turntable of horizontal angle, the adjustment is convenient, measurement accuracy is high and easy to use and spread.