29 results about "Ictiobus" patented technology

The invention relates to bacillus megaterium A-27, a microecological preparation thereof and a preparation method of the microecological preparation, the bacillus megaterium is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M 20241848, the classification name is bacillus megaterium A-27, and the Latin name is bacillus megaterium A-27. The microecological preparation is prepared by fermenting bacillus megatherium A-27. The invention also discloses a preparation method of the microecological preparation. A microecological preparation which is prepared from the bacterial strain through a corresponding method and is suitable for aquaculture acts on freshwater fishes in a feed additive mode, has broad-spectrum fish pathogenic bacterium inhibition activity, and particularly has a remarkable effect of resisting aeromonas hydrophila infection on the freshwater fishes; meanwhile, the strain can be stably colonized in freshwater fish bodies, the immunocompetence of the freshwater fishes is effectively improved, and a certain effect of promoting the growth of the fishes is achieved.

The invention provides an application of linoleic acid in preparation of a feed for relieving fish salinity stress. According to the application of the linoleic acid in preparation of the feed for relieving the salinity stress of the fishes, related experiments verify that the addition of the linoleic acid enhances the overall tolerance of the fishes to the salinity stress, and under the stress environment that the salinity continuously rises, the stress resistance of the fishes to the salinity stress is improved. Fish has better growth performance, lower physiological stress level, stronger antioxidant ability and immune response and healthier tissue structure, and is beneficial to increase of culture benefits of freshwater fish and marine fish.

The invention relates to the technical field of otolith sample preparation, in particular to a freshwater fish otolith sample preparation method which comprises the following steps: 1, sampling otolith; 2, otolith cleaning; 3, weighing the otolith; 4, fixing the otolith; 5, vacuumizing and hardening; 6, cutting the otolith; 7, fixing and cutting for the second time; 8, otolith polishing; 9, polishing the otolith; 10, otolith dyeing. According to the method, the freshwater fish otolith sample can be well prepared.

The present invention provides a method for domesticating mandarin fish using large-scaled paraloach as transit bait, and belongs to the field of freshwater fish seed breeding. The mandarin fish domestication method provided by the present invention goes through four stages: starvation → live bait feeding → transit feeding → feed, and the transit feeding of mandarin fish involved includes: fresh dead loach coated with powder → fresh dead loach coated with powder + ice-fresh loach coated with powder → ice-fresh loach coated with powder + feed. The present invention uses loach as transit bait, which not only cleverly caters to the mandarin fish's unique visual response mechanism for feeding, but also significantly improves the feeding efficiency during the feed conversion process, makes full use of the loach's flexible body shape and ability to swim quickly in the water, and perfectly matches the mandarin fish seedlings' extremely high desire to prey on dynamic and active small aquatic organisms, opening up a new efficient and environmentally friendly way for the feed domestication of mandarin fish seedlings. The domestication procedure is highly replicable, with low loss of mandarin fish fry, high domestication rate, few diseases, fast growth of mandarin fish fry and excellent quality.

The present application relates to the technical field of aquaculture, and especially relates to a high-density and high-benefit freshwater fish circulating water breeding method, which uses a high-density and high-benefit freshwater fish circulating water breeding pond, the breeding pond comprises a pond body, a water inlet pipe and a sewage suction pipe, etc.; a sewage ditch is formed on the pond body; the water inlet pipe is connected to the front side of the pond body; the sewage suction pipe is arranged on the back side of the pond body, and a plurality of branch pipes are arranged on the sewage suction pipe and located in the sewage ditch; the water inlet pipe and the sewage suction pipe are connected to an external filtering device, and a water pump is arranged on the filtering device. The present application realizes that the impurities at the bottom of the pond body are pushed backward to the sewage ditch by the scraper, and the straight plate part of the scraper blocks and limits the impurities in the process of backward movement of the scraper, so that the impurities are not lifted, the sewage passes through the water filtering port when the impurities are moved backward by the scraper, and thus the problem that the collection effect of the impurities is reduced due to the backward movement of the pond water caused by the scraper is avoided.

The invention discloses a multilayer detachable three-dimensional freshwater fish temporary rearing bin which comprises a bin body and a plurality of detachable temporary rearing bins located in the bin body, the temporary rearing bins are slidably arranged in containing grooves formed in the bin body, and the temporary rearing bins located on the same column are vertically aligned at equal intervals to serve as an experimental group. And a communicating assembly movably penetrating through each temporary rearing bin is arranged in each experimental group correspondingly. According to the freshwater fish temporary rearing device, distinguishing, temporary rearing, observation and experimental operation of freshwater fish can be achieved, circulation can be achieved, the communicating assembly is matched, fishes are prevented from entering other temporary rearing bins at the communicating position, connectivity of all the temporary rearing bins is assisted to be slowed down, great help is provided for observation of experimental operation such as virus resistance, infectivity and virus tolerance of the freshwater fish, and the survival rate of the freshwater fish is increased. The method has scientific research value and daily use value.

The invention relates to a streptomyces X-57, a microecological preparation thereof and a preparation method of the microecological preparation, the streptomyces is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M 20242239, the classification name is streptomyces X-57, and the Latin name is Streptomyces sp.X-57. The invention also discloses a preparation method of the microecological preparation, the streptomyces is preserved in the China Center for Type Culture Collection, and the strain preservation number is CCTCC NO: M 20242239. The microecological preparation is prepared by fermenting streptomycete X-57. The invention also discloses a preparation method of the microecological preparation. A microecological preparation which is prepared from the strain through a corresponding method and is suitable for aquaculture acts on freshwater fishes in a feed additive mode and has broad-spectrum activity of inhibiting fish pathogenic bacteria, and the streptomyces X-57 is used as a feed additive to feed fishes, especially crucian. The growth performance and the immunity of fishes, especially crucian carps, can be improved, and the intestinal flora can be improved. Wherein the effect of resisting infection of aeromonas hydrophila on crucian carps is particularly remarkable, meanwhile, the culture medium can be stably colonized in the crucian carps, the immunocompetence of the crucian carps is effectively improved, and the culture medium has a certain effect of promoting the growth of the crucian carps.

The invention discloses a technology for salinizing slimming fish by using mountain spring water, and relates to the technical field of fish fries, and the technology comprises the following steps: step 1, culturing fish fries, step 2, conditioning and treating wounds by using Chinese herbal medicines, and step 3, salinizing and slimming. In the step 1, various types of freshwater fishes can be bred, fish fries are put into a common fish pond for breeding, feeding is performed every day, and adult fishes are bred, and in the step 2, the adult fishes are put into a new fish pond, and medicaments are added for conditioning and preventing saprolegniasis. According to the step 3, sea salt of 5-15 DEG C is added into water according to different fish fingerlings, a circle of sea salt is evenly scattered on the periphery of the fish pond at the beginning, the sea salt is dissolved and kept for one week, water is changed after one week, the sea salt is added again, the content of the sea salt is increased by one degree at the moment, circulation is conducted in this way, one part of sea salt is added every week, the salt tolerance of the freshwater fish is increased, and the freshwater fish can appear on the market after 30-60 days. According to the present invention, the meat quality of the fish can be well changed, the fish has no mud taste and no fishy smell, the meat quality is firm, fresh and sweet, the parasites in the fish body can be completely killed, and the harmful bacteria can be reduced to the minimum.

The present invention discloses an exosome for treating lung injury, a preparation method and application thereof, and relates to the field of biotechnology. The exosome for treating lung injury is derived from aquatic animal cells; the aquatic animal is a freshwater fish; the freshwater fish include: crucian carp, grass carp, black carp, snakehead carp and catfish; the exosome for treating lung injury prepared by the present invention has multiple characteristics such as small size, evasion of phagocytosis, easy penetration of the respiratory tract endothelium layer, etc., can be applied to lung injury, can be used to prepare drugs for lung injury, regulate the lung cell repair mechanism, shorten the process of lung injury repair, and improve the lung injury repair effect.

The invention belongs to a freshwater fish breeding technology, and particularly relates to a phoxinus lagowskii large-scale breeding method which sequentially comprises the following steps of parent selection, parent breeding, artificial spawning induction, artificial insemination and juvenile fish hatching. Injecting a spawning induction agent, which is composed of 500-1000 IU of chorionic gonadotropin (HCG) per kilogram of fish body weight, 5-10 micrograms of luteinizing hormone releasing hormone analogue (LRH-A2) per kilogram of fish body weight and 1-2 mg / kg of domperidone (DOM) per kilogram of fish body weight, into the bred parent from pelvic fins; the injection dosage of the parent female fish is two times that of the parent male fish. The method solves the problems that phoxinus lagowskii parents are asynchronous in gonad development, do not lay eggs and are not concentrated in egg laying, the induced spawning rate is increased to 98% or above, and meanwhile the production time is shortened. By means of manufacturing and using the artificial fish nest, the natural hatching state of phoxinus lagowskii roes is simulated, damage to the roes in the debonding process is reduced, and the fertilization rate and hatching rate of the roes are increased to 96.8% and 92.1% respectively.

The invention relates to a streptomycete D-6, a microecological preparation thereof and a preparation method of the microecological preparation, the streptomycete D-6 is preserved in China Center for Type Culture Collection on June 13, 2024, and the preservation number is CCTCC NO: M 20241201; the classification name of the streptomyces sp.D-6 is streptomyces sp.D-6, and the Latin name of the streptomyces sp.D-6 is Streptomyces sp.D-6. The microecological preparation is prepared by fermenting the streptomycete D-6. The invention also comprises a preparation method of the streptomyces D-6 microecological preparation. The invention also comprises application of the streptomyces D-6 and the microecological preparation thereof in aquaculture. The streptomyces D-6 and the microecological preparation thereof disclosed by the invention have a relatively good antibacterial effect on common fish pathogenic bacteria such as plesiomonas shigelloides, aeromonas veronii, aeromonas anomala, shewanella xiamenensis and the like; the streptomycete D-6 is used as a feed additive to feed fishes, especially crucian carps, so that the growth performance and immunity of freshwater fishes, especially crucian carps, can be improved, and the intestinal flora of freshwater fishes, especially crucian carps, can also be improved.

The invention discloses a co-cultivation method of large-scaled paraloach and mandarin fish seedlings, and belongs to the field of freshwater fish seedling cultivation. The operation steps are as follows: 1. preparing a cultivation pond, 2. inducing spawning and hatching of mandarin fish, 3. matching breeding of large-scaled paraloach, and 4. co-cultivation of large-scaled paraloach seedlings and mandarin fish seedlings. The invention utilizes the characteristics of large-scaled paraloach that the breeding cycle is long, high-density cultivation is possible, and it is rich in nutrition to co-cultivate large-scaled paraloach seedlings and mandarin fish seedlings; the unique horizontal swimming period and morphological characteristics of large-scaled paraloach seedlings are easy to be preyed on and digested by mandarin fish seedlings, which can improve the survival rate of mandarin fish seedlings during the opening period; large-scaled paraloach seedlings are rich in nutrition and high in protein content, which promotes the rapid growth of mandarin fish seedlings, and are high-quality bait fish for mandarin fish fry to open. The invention supplies a sufficient amount of palatable bait fish when the mandarin fish opens its mouth, avoids daily catching of silver carp, bream and other fry to feed the mandarin fish fry, saves the heavy workload of manually catching bait fish fry, creatively prolongs the breeding cycle of the mandarin fish fry, and greatly increases the annual output of the mandarin fish fry.

The invention relates to a method for detecting freshwater fish ichthyophthirius multifilis, which is characterized in that a visual LAMP (loop-mediated isothermal amplification) rapid detection method is established by using 18sRNA (ribonucleic acid) gene of ichthyophthirius multifilis as a specific target gene through a specific primer, and a PCR (polymerase chain reaction) solution containing HNB dye and Bst DNA (deoxyribonucleic acid) polymerase are adopted to carry out loop-mediated isothermal amplification reaction, so as to detect the ichthyophthirius multifilis. According to the method, visual detection of ichthyophthirius multifilis can be achieved within 20 min, professional knowledge or special fluorescent dye is not needed, and whether a sample contains the ichthyophthirius multifilis or not can be judged only by observing the color of an amplification product. The method can be used for detecting ichthyophthirius multifilis of which the plasmid DNA concentration of a sample is as low as 0.01 ng / mu L, and is high in sensitivity and good in specificity. According to the method, rapid early warning can be achieved in the early outbreak stage of the ichthyophthirius multifilis, early discovery and early prevention are achieved, economic losses are reduced, and the method has wide application prospects.

A strain of Streptomyces XL-34, a microecological preparation thereof and a preparation method thereof, wherein the Streptomyces is deposited in the China Center for Type Culture Collection, the strain deposit number is CCTCC NO: M 20231087, the classification name is: novel Streptomyces XL-34, the Latin name is: Streptomyces sp. XL‑34. The microecological preparation is made by fermentation of Streptomyces XL‑34. The present invention also includes a method for preparing the microecological preparation. The microecological preparation suitable for aquaculture prepared by the strain through a corresponding method acts on freshwater fish in the form of a feed additive, has a broad spectrum of activity in inhibiting fish pathogens, and its protection rate reaches 41.67%, among which the effect on freshwater fish against infection by Aeromonas veronii is particularly significant. At the same time, it can also stably colonize in freshwater fish and effectively improve the immunity of freshwater fish, and has a certain effect on promoting fish growth.

The invention provides a pelteobagrus fulvidraco embryonic cell line as well as a subculture method and application thereof, and belongs to the field of fish cell biology. The pelteobagrus fulvidraco cell line is a pelteobagrus fulvidraco embryo-derived cell line and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: C202516. The pelteobagrus fulvidraco embryonic cell line YCE is a fibrous cell, is continuously cultured for more than 100 generations, has a good growth state, can be stably passaged, can provide a large number of pelteobagrus fulvidraco embryonic cells, can be cryopreserved, and can still be stably passaged after being unfrozen. The fish embryo source cell line disclosed by the invention not only can be used for related research on an anti-infection immune mechanism of pelteobagrus fulvidraco, but also provides a new thought for an establishment method of a freshwater fish embryo cell line, and enriches basic biological data of fishes.

The invention relates to a streptomyces Q15, a microecological preparation thereof and a preparation method of the microecological preparation, the streptomyces Q15 is preserved in China Center for Type Culture Collection on October 17, 2024, the strain preservation number is CCTCC NO: M 20242237, the classification name is streptomyces Q15, and the Latin name is Streptomyces sp.Q15. The invention also discloses a preparation method of the microecological preparation, the streptomyces Q15 is preserved in China Center for Type Culture Collection on October 17, 2024, and the strain preservation number is CCTCC NO: M 20242237. The microecological preparation prepared by fermenting streptomyces Q15 is suitable for aquaculture, and can effectively inhibit various freshwater fish pathogenic bacteria such as aeromonas hydrophila, aeromonas veronii, edwardsiella tarda and the like; the broad-spectrum fish pathogenic bacterium inhibition activity is realized; the strain can be stably colonized in freshwater fish bodies, is harmless to fishes, and can enhance the immunity of the freshwater fish, so that the resistance of the freshwater fish such as crucian and the like to fish pathogenic bacteria is remarkably improved.

The application discloses a bacillus megaterium strain for increasing adversity tolerance of aquatic animals and application thereof. The bacillus megaterium strain is named bacillus megaterium SCAU-Ohb1, the strain has been preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO.62119, and the preservation date is December 13, 2021. The bacillus megaterium SCAU-Ohb1 is isolated from fish intestinal tracts and is safe to fish bodies. After the fermentation liquor of the bacillus megaterium SCAU-Ohb1 is mixed with a basic daily ration and is used for feeding freshwater fish, the activity of important antioxidant enzymes in the fish bodies can be significantly improved, the salt tolerance of gill tissues of the freshwater fish under acute salt stress can be increased, and the survival rate of the fish bodies can be improved. The application provides a new method for improving adversity tolerance of aquatic animals, has important significance for the aquaculture industry, and is expected to improve production efficiency and reduce losses.

The invention relates to the technical field of biology, in particular to a primer group, a kit and a detection method for detecting freshwater fish in a water environment. The invention provides a primer group for detecting freshwater fish in a water environment. The sequence of the primer group is as shown in SEQ ID NO: 1-SEQ ID NO: 2. The macro barcode primer group provided by the invention can improve the accuracy of freshwater fish resource monitoring, effectively improve the accuracy of freshwater fish environment diversity detection, and meet the demand of accurate monitoring of freshwater fish resources in actual ecological detection work.

The invention provides a salt-tolerant bacillus for freshwater fish culture. The preservation number of the salt-tolerant bacillus is CGMCC (China General Microbiological Culture Collection Center) No.32389. The salt-tolerant bacillus CAG2307-32 strain provided by the invention has no pathogenicity to crucian carps, can be widely applied to culture production of freshwater fishes such as crucian carps and the like, can effectively regulate and control water quality, promote fish growth and enhance antioxidant and immune levels of fish bodies, and has great application potential.

The present invention discloses a method for artificially controlling off-season breeding of mandarin fish, which relates to the field of freshwater fish breeding technology. The method comprises the following steps: (1) screening mandarin fish broodstock and transferring them to indoor broodstock cultivation barrels for cultivation; (2) controlling the indoor ambient temperature so that the water temperature in the cultivation barrels for male and female mandarin fish is maintained at 13°C for 6 weeks, and then controlling the water temperature to rise to 23°C within 2 weeks and maintain it for 4 weeks. The present invention promotes the early development and maturation of the male fish's gonads through cooling and heating treatments, and promotes the early development and maturation of the female fish's gonads through cooling and heating treatments and full-process blue LED light source irradiation treatments, allowing subsequent artificial induction of labor and artificial insemination operations. Compared with traditional breeding methods, this method has a higher degree of controllability, better development of the male fish's gonads, and earlier ovulation of the female fish, enabling mandarin fish to be put on the market faster and better supplied to the market that same year, and has extremely high development potential.

The invention discloses a running water net cage culture method for coreius fasciatus. The running water net cage culture method comprises the following steps: S1, setting a culture environment and a net cage; s2, fingerling stocking; s3, feeding management; s4, daily management; s5, disease prevention and control; s6, harvesting and transporting, in the step S1, a plurality of net cages are prepared, the net cages need to be placed in a pond or a reservoir with the water depth larger than 4 m, the pond or the reservoir needs good water quality and high dissolved oxygen, every three net cages form a group, a water pushing machine is arranged on one side of one group of net cages, a gas compressor is arranged on the inner side of the water pushing machine, and a baffle is arranged in the water pushing machine. An exclusive breeding mode different from conventional freshwater fishes is provided for coreius guichenoti, the overall design of water flowing and environment regulation is emphasized, and the feeding activity and growth efficiency of coreius guichenoti are improved by improving the breeding environment directly aiming at the core problems of insufficient water exchange, lack of water flow stimulation and the like in still water breeding. And a complete scheme is provided for efficient and healthy coreius coreius culture.

The invention belongs to the technical field of pathogenic microorganism screening and aquaculture disease prevention and control, and discloses an aeromonas salmonicida natural attenuated strain derived from channel catfish and application of the aeromonas salmonicida natural attenuated strain, and the preservation number of the strain is CCTCC NO: M20252718. Through artificial infection, the toxicity of the strain on tested rainbow trout and grass carp is far lower than that of an aeromonas salmonicida virulent strain, and a live vaccine prepared from the strain is used for performing immune protection on rainbow trout and grass carp, so that bacterial diseases caused by the aeromonas salmonicida virulent strain can be effectively prevented. In the culture process, the strain can effectively reduce the use of antibiotic drugs, and has a positive promotion effect on reducing drug residues of freshwater fishes and further realizing green culture.

The invention belongs to the field of animal model construction, and particularly relates to a construction method and application of an early embryonic development cataract eye disease animal model. The invention finds that acute exposure of N-(1, 3-dimethylbutyl)-N '-phenyl-p-phenylenediamine (6PPD) and polystyrene microspheres (PS) in an embryonic development stage can cause eye dysplasia and visual dysfunction in a zebra fish larva stage, and the zebra fish has typical lens opacification-like cataract pathological characteristics; meanwhile, the phenomena of phototactic behavior inhibition, eye cell death increase and the like occur. The 6PPD / PS treatment interferes with visual perception and phototransduction pathways, significantly affects photoreceptor outer segment, ferroptosis, endocytosis, retinoic acid metabolism and other pathways, and can enhance the activation of ferroptosis regulation genes by enhancing oxidative stress and mitochondrial dependent apoptosis of eye tissues, thereby aggravating the cataract process. The invention finds that cataract can be generated through the 6PPD / PS effect in the development process of freshwater fish vertebrates for the first time, and provides model support for research on pathogenesis and intervention strategies of the freshwater fish vertebrates.

The invention provides an establishment method and application of a pseudorasbora parva snout-end cell line, and belongs to the technical field of freshwater fish cell culture.The establishment method comprises the following steps that (1) primary culture is conducted, specifically, pseudorasbora parva snout-end tissue is digested through collagenase II, a cell / tissue suspension is prepared and inoculated into a culture bottle subjected to polylysine coating treatment, and a culture medium is obtained; culturing by using a primary culture medium; (2) subculture: when the cell generations are 1-20 generations, culturing by using a primary culture medium which contains an epidermal growth factor, a chick embryo extract and 20% fetal calf serum; after more than 20 generations, a subculture medium is used for culture, and the subculture medium contains 10% of fetal calf serum and does not contain epidermal growth factors and chick embryo extracts; and (3) collecting passage cells to obtain the pseudorasbora parva snout end cell line. The pseudorasbora parva snout end cell line established by the method can realize continuous passage and proliferation, can be recovered after ultralow temperature cryopreservation, and can be applied to the fields of exogenous gene expression research, water environment pollutant monitoring and the like.

The invention discloses a throwing-type throwing device for freshwater fish culture, and relates to the technical field of fish culture, the throwing-type throwing device comprises a culture block, the upper surface of the culture block is also provided with a protective barrier, the upper surface of the culture block is also provided with a throwing mechanism, the throwing mechanism comprises a batch feeder, and the surface of one side of the batch feeder is fixedly connected with a feeding box and a conveying mechanism; the material conveying mechanism comprises an electric telescopic rod, a center wedge block is arranged on the upper surface of the electric telescopic rod, the material distributing mechanism is composed of a guiding assembly and a shaking assembly, the guiding assembly comprises a material gathering rod and a material dispersing rod, the material gathering rod and the material dispersing rod are located on the upper surfaces of the two sides of the protective barrier correspondingly, and the shaking assembly comprises two rotating seats; according to the scheme, by arranging the material conveying mechanism, the material distributing mechanism and the throwing mechanism, the distribution position of feed can be adjusted in an auxiliary mode according to the characteristics of the fed feed in the using process, and the feeding efficiency of fishes is improved.

The invention discloses a green field fish fin ray cell line as well as a construction method and application thereof, and belongs to the technical field of freshwater fish cell culture, the green field fish fin ray cell line is named as green field fish fin ray cell line CQF, and is preserved in China Center for Type Culture Collection on April 2, 2025, and the preservation number is CCTCC NO: C202591. The cell line is derived from green field fish, and on the basis of fin rays of the green field fish, the culture method comprises the following steps: selecting the green field fish with excellent health conditions, temporarily rearing at room temperature for 2 weeks, feeding once in the morning and once in the evening, simultaneously preparing a primary culture medium and a subculture medium, treating fin ray tissues, performing primary culture on fin ray tissue cells, performing subculture, and performing cell cryopreservation and recovery. The green field fish fin ray cell line provided by the invention has the advantages of strong division ability, fast growth speed, good stability, strong repeatability, simple and easy operation, and high recovery activity, and can establish a fish hypoxia stress model as a new in vitro research tool, accelerate the breeding process of good varieties, and improve the breeding efficiency.

The utility model discloses a freshwater fish feed feeding device capable of discharging feed dispersedly, relates to the technical field of feeding devices, and aims to solve the problems that in the feeding process of an existing feeding device, due to the fact that the mode that the feeding device moves along a moving track for concentrated feeding is adopted, feed feeding is not dispersed enough and not uniform, repeated feeding is needed, and the feeding efficiency is high. The feeding device comprises a primary feeding table and a secondary feeding table, the secondary feeding table is located below the primary feeding table, a connecting plate is arranged between the secondary feeding table and the primary feeding table, the secondary feeding table and the primary feeding table are connected through the connecting plate, a throwing assembly is arranged on the surface of the primary feeding table, and the throwing assembly is arranged on the surface of the primary feeding table. The throwing assembly is arranged above the primary feeding platform and comprises a throwing rod and a feeding basin, the dispersing assembly is arranged above the secondary feeding platform and comprises a feed barrel and a funnel, the throwing assembly is borne by the primary feeding platform, the dispersing assembly is borne by the secondary feeding platform, and the primary feeding platform and the secondary feeding platform are connected through a connecting plate.

The application provides a salt-tolerant bacillus for freshwater fish culture, and the preservation number is CGMCC No.32389.The salt-tolerant bacillus CAG2307-32 strain provided by the application has no pathogenicity to crucian carp, can be applied in a large amount in the freshwater fish culture production such as crucian carp, can effectively regulate water quality, promote fish growth, and enhance the oxidation resistance and immune level of fish, and has great application potential.

The invention belongs to the technical field of ecological environmental protection, and discloses a freshwater fish habitat protection effect and priority evaluation method in a protection area, and the method comprises the steps: employing the collinearity of VIF test variables, employing a MaxEnt algorithm to construct a species distribution model of each species in global sea river migration fishes, and simulating the habitat distribution based on a habitat model; according to the performance of the AUC and TSS evaluation model, simulation of the model is realized; according to the distribution information of the protection area, the stability of the sea-river migratory fish habitats outside and inside the protection area and the planning priority of the protection area are evaluated respectively. According to the method, the protection effect of the land protection area on the freshwater fish habitat and the protection area planning priority for the freshwater fish habitat are evaluated respectively, and the unbalance problem that in the prior art, freshwater fishes are often ignored in the protection area setting process is solved.