33 results about "Ictiobus" patented technology

The invention discloses a fermented low-salt semi-dried tilapia mossambica, a processing method thereof and application thereof. The processing method comprises the steps of: removing the internal organs and the scales of the tilapia mossambica; cutting along backside or abdomen; overturning; flatly pressing to form a symmetrical tilapia mossambica piece except the tail of the tilapia mossambica;washing with cleaning water; washing with salt water with 1%-4% of concentration to remove fishy smell and obtain the tilapia mossambica piece without the fishy smell; paving salt on the surface of the tilapia mossambica piece without the fishy smell, wherein the weight of the salt is equal to 3-8% of that of the tilapia mossambica; sousing for 8-24 hours; drying; or soaking for 8-24 hours with 5-10% of salt water to obtain the low-salt tilapia mossambica piece; spraying zymophyte on the surface of the tilapia mossambica piece, wherein the inoculum size of the zymophyte is 105-107 CFU / g fish meat; fermenting for 10-48 hours in a sealing way under the temperature of 12-20 DEG C; and drying the fermented tilapia mossambica piece to obtain the fermented low-salt semi-dried tilapia mossambicawith 30-60% of water content. The product has lubricating meat quality and typical flavor of dried meat. The processing method can be adapted to processing freshwater fishes.

The invention discloses a disease-resistant feed additive for freshwater fishes and a preparation method thereof. The disease-resistant feed additive is prepared from the following raw materials by weight percent: 0.3-0.4% of allicin, 0.05-0.15% of vitamin C, 15-20% of salt, 35-45% of zeolite powder, 25-35% of fulvic acid, 7.5-10.5% of a Chinese medicine extract, and 7-13% of probiotics powder, wherein the Chinese medicine extract include 20-40% of a rhubarb extract, 20-40% of a rhizoma coptidis extract, and 20-40% of a radix scutellariae extract. The disease-resistant feed additive disclosed by the invention can effectively overcome the diseases such as gill rot diseases, fin-rot diseases, skin ulcerative diseases, bleeding diseases, red skin diseases and body surface festers of freshwater fishes, and then replace antibiotics.

The invention discloses universal metabarcoding amplification primers for freshwater fish mitochondria 12S and an application method thereof, and belongs to the field of biotechnology. The 16 metabarcoding amplification primers are designed according to the gene sequences of common freshwater fish mitochondria 12S, including 6 upstream primers and 10 downstream primers; the primer pairs have widecoverage on fish communities and high amplification efficiency, the primer combinations can amplify products with the length of 70bp to 350bp and are compatible with different high-throughput sequencing platforms, the multi-primer combination method can reduce non-specific amplification and increase the PCR success rate, and the primers can be widely applied to fish species identification, fish diversity analysis and other research as characteristic barcode sequences.

The invention relates to an automatic collection device for semi-floating fish eggs. The automatic collection device is characterized in that the automatic collection device comprises a right-angle bent pipe, a suspension rope, a net bag and an inflating pipe, the right-angle bent pipe is suspended in an egg laying pond through the suspension rope and the suspension rope is tied at the bent position of the right-angle bent pipe; the right-angle bent pipe comprises a vertical pipe section and a transverse pipe section, a weight is arranged at the lower end of the vertical pipe section to keep the vertical pipe section to be in a vertical state when the right-angle bent pipe is in a suspended state; the mouth of the net bag is sheathed at the rear end of the transverse pipe section and is tightly tied through a tying rope; and the inflating pipe is inserted into the vertical pipe section by certain length from the bent position of the right-angle bent pipe. The automatic collection device for the semi-floating fish eggs has the advantages that the device is simple, convenient and feasible to operate, the manufacturing cost is low, the labor intensity can be reduced and the working efficiency is improved; the damage to the fish eggs is reduced, the collection rate of the fish eggs is high and the hatching rate of fish fries is high; and the structure is simple, the space of the egg laying pond is not occupied, the fish egg collection and the inflation can be conducted at the same time, additional investment is not required and the automatic collection device is particularly suitable for the artificial breeding of seawater and freshwater fishes such as four major domesticated fish and the like which lay semi-floating eggs.

The invention relates to streptomyces lateritius Z1-26, a microecological preparation and a preparation method of the microecological preparation, the streptomyces lateritius Z1-26 is preserved in the China Center for Type Culture Collection, and the strain preservation number is CCTCC NO: M 2021705. The microecological preparation prepared by fermenting the streptomyces lateritius Z1-26 is suitable for aquaculture, acts on freshwater fishes such as crucian carps, grass carps and carps in the form of a feed additive, has the protection rate of 60%, particularly has a remarkable effect of resisting infection of aeromonas hydrophila on the freshwater fishes, and can be used as a feed additive for aquaculture.Meanwhile, the growth performance and the immunocompetence of the freshwater fishes can be effectively improved.

The invention relates to an artificial propagation and roe incubation method for freshwater fishes, in particular to an artificial propagation and roe incubation method for schizopygopsis malacanthus, and belongs to the technical field of artificial propagation and roe incubation of fishes. The artificial propagation and roe incubation method for schizopygopsis malacanthus mainly comprises the steps of parent fish selection, spawning induction, artificial impregnation, roe incubation and fry raising. The artificial propagation and roe incubation method for schizopygopsis malacanthus has the advantages that the fertilization rate can reach 89.6%, the incubation rate can reach 95%, and the problem of resource scarcity caused by environmental problems is resolved; the fry incubation process is convenient to conduct, the controllability is improved, and requirements for manpower and management in the incubation process are lowered.

The invention provides a novel PCR (Polymerase Chain Reaction) primer (16s 200) for identifying or assisting in identifying freshwater fish species. A nucleotide sequence of the primer is SEQ ID NO.1 in a sequence table. The PCR primer is applied to eDNA metabolism coding of a fish mitochondrial DNA sequence. The PCR primer is adopted to perform PCR amplification on eDNA in a water body, based on a high-throughput sequencing method, the problem that an effective species specific primer is difficult to obtain under the condition that various closely-related species exist in the water body can be effectively solved, and based on an environmental DNA monitoring technology, an effective technical support is provided for taxonomy research and diversity protection of fishes. The method for identifying the freshwater fish species by using the PCR primer has the advantages of short consumed time, simplicity and convenience in operation, high specificity and the like.

The invention discloses an automatic whole-pond balanced bait feeding system and method for freshwater fish culture, and the system is characterized in that a bait flow guide pipe body is fixedly connected to a culture pond; the water driving module is provided with a water suction end and a water discharge end, the water suction end is arranged in the culture pond, and the water discharge end is communicated with the bait flow guide pipes; the feeding structure is communicated and fixedly connected to the bait flow guide pipe body; the airflow guide pipe bodies are evenly and fixedly connected to the culture pond, and the airflow guide pipe bodies are arranged under the airflow guide pipe bodies in a right opposite mode. The air inflation and exhaust module is fixedly connected to the installation ground and communicates with the airflow guide pipe body, and air inflation and exhaust are conducted on the airflow guide pipe body through the air inflation and exhaust module. The discharging structure is fixedly connected between the bait flow guide pipe body and the airflow guide pipe body. The technical problems that according to a feeding mode of freshwater fish culture bait, the feeding range is limited, efficiency is low, labor intensity is high, bait throwing is locally concentrated and uneven, regular and quantitative feeding is difficult, and consequently freshwater fish culture is difficult to achieve the expected effect are solved.

The invention relates to the field of genetic engineering, in particular to a low-temperature neutral histidine phytase APPA206, gene thereof, a recombinant carrier comprising the gene and application. The amino acid sequence of the low-temperature neutral histidine phytase APPA206 is shown as SEQ ID NO.1, and the invention also provides the gene appa 206 which encodes the low-temperature neutral histidine phytase. The low-temperature neutral histidine phytase is obtained, has high enzymatic activity at the pH range of 4.5 to 8.5, and can resist hydrolysis of trypsin in an aquatic animal body. The characteristics are suitable for a freshwater fish feed.

The invention discloses a bulking machine for ready-to-eat fish and shrimp foods. The bulking machine not only can be used for processing small-scale fishes and shrimps belonging to marine products but also can be used for processing freshwater fishes, and products are soft and delicious and are suitable for old people and young people. The bulking machine comprises an electric heating part and a mechanical pressurizing part; a solid-state relay, a leakage protector and an intelligent temperature controller are mounted in a casing; a front base and a rear base are arranged in the casing, a basic fixed frame is mounted on the front base and the rear base, a load-bearing guard plate is mounted at the front end of the basic fixed frame, and a load-bearing bracket is mounted at the rear end of the basic fixed frame; a lower pressure block in which a lower heater is arranged is mounted in the load-bearing guard plate; an upper pressure block is hinged to the front part of the load-bearing bracket through a lower pin shaft of a back-swinging connecting rod, wherein an upper heater is arranged in the upper pressure block, and the back-swinging connecting rod is arranged at the rear part of the upper pressure block; a pressure handle is hinged to the rear part of the load-bearing bracket through a pin shaft of a pressure connecting rod by the pressure connecting rod at the tail end of the pressure handle, and middle connecting rods are hinged to the back-swinging connecting rod and a rotating arm of the pressure connecting rod. The bulking machine disclosed by the invention is low in manufacturing cost, products are convenient to eat, consumption groups are extensive, and the bulking machine is especially suitable for small-scale producers to popularize and use.

The invention discloses a fish body dissection and cleaning device based on freshwater fish processing. The device comprises a frame, a cleaning box, a second rotating rod and a dirt collecting box. An electric motor is fixed to the frame by bolts; a fixed rod is welded to the frame; the electric motor is in keyed joint with a first rotating rod; both ends of the first rotating rod are movably connected to the frame by a ball rotating bearing; the first rotating rod is welded with blades; the fixed rod passes through a fixing seat; a first cavity and a second cavity are opened in the inner wall of the fixing seat; the side wall surface of the top of the fixing seat is sunken inwardly to form a groove; and the top end of the fixing seat is provided with a clamping board. According to the fish body dissection and cleaning device based on freshwater fish processing, the fish bodies are not polluted during the cleaning process, thereby improving the cleaning effect; and the cut-off fish bodies, fish heads and fish tails can be classified and collected for facilitating subsequent processing.

The invention discloses a freshwater fish identification method based on improved VGGNet, and relates to the technical field of fish identification. The method comprises the following steps: performing preprocessing and data enhancement on fish images in a freshwater area, expanding an existing data set, performing network structure improvement on VGGNet, selecting a small convolution kernel as a convolution kernel of a convolution layer, selecting a maximum pooling kernel parameter for keeping the same pooling kernel parameters of each pooling layer, performing classification by adopting a three-layer full-connection layer and then connecting Softmax, and performing classification by adopting a three-layer full-connection layer; and training and verifying a model according to the data set by using an improved VGGNet, and performing freshwater fish identification through the model.

The invention belongs to the technical field of freshwater fish breeding, and discloses an artificial breeding method of spinibarbus hollandi. The artificial breeding method comprises the following steps of parent fish rearing, artificial spawning induction, artificial insemination, fertilized egg hatching and fry rearing. By conducting artificial breeding on the spinibarbus hollandi, the spawning induction rate reaches 80% or above, the fertilization rate reaches 90% or above, the fry survival rate reaches 90% or above, efficient artificial fry rearing of the spinibarbus hollandi is achieved, the production efficiency is improved, and the sustainable development and utilization of the parent fish are ensured.

The invention provides a three-dimensional freshwater fish germplasm resource protection reef which comprises at least three sedimentation pipes, wherein the multiple sedimentation pipes are arranged at intervals, any two adjacent sedimentation pipes are connected together through a plurality of supporting rods, all the sedimentation pipes and the supporting rods are connected to form a reef cage which is hollow up and down, coir ropes are wound on the peripheral surface of the reef cage, and a plurality of scallops are fixedly connected to the coir ropes. By the adoption of the technical scheme, smooth water flowing is facilitated, the blocking effect on water flow and silt is reduced, a large number of aquatic animals and plants are promoted to be attached to the artificial reef to grow and breed, fish germplasm resources near the reef are protected, and an ecological system is restored.

The invention relates to an automatic collection device for semi-floating fish eggs. The automatic collection device is characterized in that the automatic collection device comprises a right-angle bent pipe, a suspension rope, a net bag and an inflating pipe, the right-angle bent pipe is suspended in an egg laying pond through the suspension rope and the suspension rope is tied at the bent position of the right-angle bent pipe; the right-angle bent pipe comprises a vertical pipe section and a transverse pipe section, a weight is arranged at the lower end of the vertical pipe section to keep the vertical pipe section to be in a vertical state when the right-angle bent pipe is in a suspended state; the mouth of the net bag is sheathed at the rear end of the transverse pipe section and is tightly tied through a tying rope; and the inflating pipe is inserted into the vertical pipe section by certain length from the bent position of the right-angle bent pipe. The automatic collection device for the semi-floating fish eggs has the advantages that the device is simple, convenient and feasible to operate, the manufacturing cost is low, the labor intensity can be reduced and the working efficiency is improved; the damage to the fish eggs is reduced, the collection rate of the fish eggs is high and the hatching rate of fish fries is high; and the structure is simple, the space of the egg laying pond is not occupied, the fish egg collection and the inflation can be conducted at the same time, additional investment is not required and the automatic collection device is particularly suitable for the artificial breeding of seawater and freshwater fishes such as four major domesticated fish and the like which lay semi-floating eggs.

The invention belongs to the technical field of fish production estimation and discloses a method for predicting the production of freshwater cultured fish, which can realize accurate prediction of the production of freshwater cultured fish. Collect historical meteorological data and historical freshwater aquaculture fish production data, and use stepwise regression analysis to screen meteorological factors that have a significant impact on freshwater aquaculture fish production. In order to judge whether to introduce or delete new variables without changing the overall significance of the equation, Therefore, a coefficient is set to distinguish the introduction and deletion of new variables. Using these significant meteorological factors to establish a forecasting model for predicting freshwater aquaculture production, the use of this model can provide assistance for the production management of freshwater fish aquaculture.

A strain of Bacillus Velez, its microecological preparation and its preparation method, the Bacillus Velez is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M 2019218, and the classification name is: Velez Bacillus BvL03, Latin scientific name: Bacillus velezensis BvL03. The microecological preparation suitable for aquaculture made by this strain has a broad-spectrum inhibitory effect on fish pathogenic bacteria through feed addition, water splashing or intraperitoneal injection, and the protection rate reaches 85%. After acting on fish, it can effectively It has the best protective effect against the infection of fish pathogens such as Aeromonas hydrophila on grass carp, crucian carp and other freshwater fish, especially grass carp.

The invention discloses a fermented low-salt semi-dried tilapia mossambica, a processing method thereof and application thereof. The processing method comprises the steps of: removing the internal organs and the scales of the tilapia mossambica; cutting along backside or abdomen; overturning; flatly pressing to form a symmetrical tilapia mossambica piece except the tail of the tilapia mossambica;washing with cleaning water; washing with salt water with 1%-4% of concentration to remove fishy smell and obtain the tilapia mossambica piece without the fishy smell; paving salt on the surface of the tilapia mossambica piece without the fishy smell, wherein the weight of the salt is equal to 3-8% of that of the tilapia mossambica; sousing for 8-24 hours; drying; or soaking for 8-24 hours with 5-10% of salt water to obtain the low-salt tilapia mossambica piece; spraying zymophyte on the surface of the tilapia mossambica piece, wherein the inoculum size of the zymophyte is 105-107 CFU / g fish meat; fermenting for 10-48 hours in a sealing way under the temperature of 12-20 DEG C; and drying the fermented tilapia mossambica piece to obtain the fermented low-salt semi-dried tilapia mossambicawith 30-60% of water content. The product has lubricating meat quality and typical flavor of dried meat. The processing method can be adapted to processing freshwater fishes.

The invention discloses a feed for improving feed conversion efficiency and protein deposition rate of freshwater fish and its application. The feed includes: white fish meal, casein, soybean oil, fish oil, cornstarch, vitamin premix, and chole chloride Alkali, mineral salt premix, CMC (hydroxymethylcellulose) and cellulose. Although the feed of the present invention has a low content of fishmeal, the feed conversion efficiency is still very high, which reduces the cost of aquaculture feed, and the freshwater fish fed with the feed provided by the present invention can effectively reduce the fat deposition rate of the fish body and reduce the liver-to-body ratio. Good for liver health.

The invention discloses a Chinese herbal medicine additive for preventing and treating bacterial body rot of scaleless fish and application. The Chinese herbal medicine additive comprises the following components in parts by weight: 0.1-1.5 parts of a Chinese herbal medicine mixture, 0.2-1.0 part of organic acid and 0.03-0.1 part of vitamin K, wherein the Chinese herbal medicine mixture comprises the following components in parts by weight: 20-25 parts of dandelion, 20-25 parts of wormwood, 15-20 parts of houttuynia cordata, 15-20 parts of honeysuckle and 15-20 parts of cinnamon. The Chinese herbal medicine additive provided by the invention is especially suitable for preventing and treating bacterial body rot of scaleless fishes, and is especially suitable for scaleless freshwater fishes such as channel catfish, pelteobagrus fulvidraco, clarias fuscus and silurus meridionalis. The use of antibiotics can be reduced, the bacteriostasis and sterilization performance is improved, the fry immunity is improved, and the fry growth is promoted.

The invention belongs to the technical field of aquaculture and discloses antibacterial peptide YcHbeta. An amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The antibacterialpeptide can be used for killing lchthyophthirius parasitizing in freshwater fishes in vitro. Experiments prove that the antibacterial peptide ycHbeta is a natural immune substance from pelteobagrus skin mucus, is nontoxic and harmless to aquatic animals, has high membrane penetrability, is effective on different life history stages of lchthyophthirius and can easily kill trophozoite and predators;thick cysts at a cystic stage can be damaged by using high-concentration antibacterial peptide YcBbeta for treatment, so that wrapped filial-generation polypide can be dissolved.

A processing method for squid, squid, bighead carp and silver carp containing omega-3 thornless fish belongs to the field of aquatic product fish processing; the method is to process squid, squid and freshwater fish containing the first and second omega-3 in seafood fish The first and second bighead carp and carp body parts are prepared separately; bighead carp blood, squid meat and bighead carp's heart, liver, kidney, skin and fins are added together to make dark surimi; Squid meat, bighead carp meat, boiled intestine oil, glue made from scales, finely ground stinging fish bladder are added together to make light-colored surimi and put into fish-shaped molds, then add dark-colored surimi to embellish it, and send it to the microwave oven Cooked into a boneless fish rich in omega-3.

The invention discloses a processing method for enhancing chewiness of fish meat by using a biological method, which comprises the following steps: pretreating fish, draining water, smearing a composite biological fermentation agent which is 1-2% of the mass of the fish, and fermenting the fish at low temperature for 1.5-2.5 days, and taking out the fermented fish, wherein the compound biological leavening agent is prepared by mixing pediococcus pentosaceus, lactobacillus sake and staphylococcus carnosus according to the mass ratio of 1: 1: 3, and dissolving the mixture in water according to the mass ratio of 1:(10-20); and adding 2-3% of table salt, 0.03-0.05% of ginger powder and 0.03-0.05% of licorice powder based on the mass of the fish into the water. According to the processing method disclosed by the invention, the product is endowed with fragrant and sweet taste and chewy mouth feel, so that the single mouth feel and quality of the traditional frozen or hot-processed fish are changed; the invention also solves the problem of fishy smell of fishes, especially mud fishy smell of freshwater fishes; the product is chewy and elastic, has no bad taste, and is a nutritious food suitable for people of all ages.

The invention relates to the technical field of genetic engineering of freshwater fish, and discloses an isolated carp antiviral protein Pdcd6ip and its antiviral activity. The nucleotide sequence of the gene of the carp antiviral protein Pdcd6ip is 1-2610bp in SEQ ID NO:1 The sequence shown, the encoded amino acid sequence is the sequence shown in SEQ ID NO:2. Tests show that the expression of the Pdcd6ip protein cloned by the present invention can affect the proliferation of carp spring viremia virus (SVCV), and the Pdcd6ip protein plays an important role in the process of cell defense against SVCV infection, and has a low impact on the cell activity of ordinary cells . The gene or protein of the invention provides a new target for preparing anti-SVCV medicines.

A strain of Streptomyces alginolyticus N1-32, its microecological preparation and its preparation method. The Streptomyces alginolyticus N1‑32 is preserved in the China Center for Type Culture Collection, and the strain preservation number is CCTCCNO: M 2019381. The Streptomyces alginolyticus N1‑32 can effectively inhibit the growth of common fish pathogens, especially Aeromonas victoria. The probiotics of Streptomyces alginolyticus N1‑32 acted on liver cells of grass carp showed that the bacteria had no toxic effect on fish. By using the probiotics of Streptomyces alginolyticus N1‑32 as a feed additive to feed freshwater fish such as grass carp and crucian carp, the results show that the growth rate and bait efficiency of grass carp are improved, and the immunity of freshwater fish can be enhanced at the same time, thus Significantly improve the resistance of grass carp and other freshwater fish to fish pathogenic bacteria.

The invention belongs to the technical field of storage and transportation of aquatic products. The invention discloses a method for relieving freshwater fish transportation stress. The method is characterized by comprising the following steps of, 1) preparing a fermentation substrate, specifically, mixing astragalus membranaceus, ginseng, angelica sinensis, wolfberry fruits and radix ophiopogonisto obtain the fermentation substrate; 2) preparing a culture solution; 3) preparing inoculation liquid, specifically, selecting microorganisms according to a volume ratio of lactobacillus acidophilusto lactobacillus plantarum to bifidobacterium to streptococcus thermophilus of (1-4): (1-3): (1-6): (1-8); 4) treating the fermentation substrate obtained in the step 1); 5) fermenting; 6) adjustingthe pH value of extracted fermentation liquor to 7.0 by adopting sodium carbonate after the fermentation is finished, and filtering to obtain filtrate, specifically, the final fermentation liquor; and(7) adding the fermentation liquor obtained in the step (6) into a transportation water body of the freshwater fish. The method is natural and harmless, the survival rate of freshwater fish in the transportation process is increased, the loss of muscle nutrient components of fish body is reduced, oxidative damage of the fish body is relieved, and muscle quality is guaranteed to a great extent.

The invention provides a method for constructing a bottom-layer fish ecological reef in a fresh water basin. The method comprises the following steps of respectively fixing scallop or oyster shells ona plurality of coir ropes to form a fish inhabiting unit, binding one end of the fish inhabiting unit on a floating ring, and outwards extending the other end of the fish inhabiting unit, and enabling the floating ring and the fish inhabiting unit to form the ecological reef; and putting the ecological reef into a fry collecting water area to quantitatively gather low-layer fish fries, so that the construction cost of the ecological reef is reduced, the ecological reef can quickly settle to a designated place, and the gathering effect of low-layer fishes is improved. Powerful biocenosis is conveniently formed, the stability of an ecological reef system is enhanced, and growth, development and propagation of freshwater fishes are facilitated.