12 results about "Ictiobus" patented technology

The present application relates to the technical field of aquaculture, and especially relates to a high-density and high-benefit freshwater fish circulating water breeding method, which uses a high-density and high-benefit freshwater fish circulating water breeding pond, the breeding pond comprises a pond body, a water inlet pipe and a sewage suction pipe, etc.; a sewage ditch is formed on the pond body; the water inlet pipe is connected to the front side of the pond body; the sewage suction pipe is arranged on the back side of the pond body, and a plurality of branch pipes are arranged on the sewage suction pipe and located in the sewage ditch; the water inlet pipe and the sewage suction pipe are connected to an external filtering device, and a water pump is arranged on the filtering device. The present application realizes that the impurities at the bottom of the pond body are pushed backward to the sewage ditch by the scraper, and the straight plate part of the scraper blocks and limits the impurities in the process of backward movement of the scraper, so that the impurities are not lifted, the sewage passes through the water filtering port when the impurities are moved backward by the scraper, and thus the problem that the collection effect of the impurities is reduced due to the backward movement of the pond water caused by the scraper is avoided.

The invention discloses a multilayer detachable three-dimensional freshwater fish temporary rearing bin which comprises a bin body and a plurality of detachable temporary rearing bins located in the bin body, the temporary rearing bins are slidably arranged in containing grooves formed in the bin body, and the temporary rearing bins located on the same column are vertically aligned at equal intervals to serve as an experimental group. And a communicating assembly movably penetrating through each temporary rearing bin is arranged in each experimental group correspondingly. According to the freshwater fish temporary rearing device, distinguishing, temporary rearing, observation and experimental operation of freshwater fish can be achieved, circulation can be achieved, the communicating assembly is matched, fishes are prevented from entering other temporary rearing bins at the communicating position, connectivity of all the temporary rearing bins is assisted to be slowed down, great help is provided for observation of experimental operation such as virus resistance, infectivity and virus tolerance of the freshwater fish, and the survival rate of the freshwater fish is increased. The method has scientific research value and daily use value.

The invention discloses a technology for salinizing slimming fish by using mountain spring water, and relates to the technical field of fish fries, and the technology comprises the following steps: step 1, culturing fish fries, step 2, conditioning and treating wounds by using Chinese herbal medicines, and step 3, salinizing and slimming. In the step 1, various types of freshwater fishes can be bred, fish fries are put into a common fish pond for breeding, feeding is performed every day, and adult fishes are bred, and in the step 2, the adult fishes are put into a new fish pond, and medicaments are added for conditioning and preventing saprolegniasis. According to the step 3, sea salt of 5-15 DEG C is added into water according to different fish fingerlings, a circle of sea salt is evenly scattered on the periphery of the fish pond at the beginning, the sea salt is dissolved and kept for one week, water is changed after one week, the sea salt is added again, the content of the sea salt is increased by one degree at the moment, circulation is conducted in this way, one part of sea salt is added every week, the salt tolerance of the freshwater fish is increased, and the freshwater fish can appear on the market after 30-60 days. According to the present invention, the meat quality of the fish can be well changed, the fish has no mud taste and no fishy smell, the meat quality is firm, fresh and sweet, the parasites in the fish body can be completely killed, and the harmful bacteria can be reduced to the minimum.

The invention relates to a method for detecting freshwater fish ichthyophthirius multifilis, which is characterized in that a visual LAMP (loop-mediated isothermal amplification) rapid detection method is established by using 18sRNA (ribonucleic acid) gene of ichthyophthirius multifilis as a specific target gene through a specific primer, and a PCR (polymerase chain reaction) solution containing HNB dye and Bst DNA (deoxyribonucleic acid) polymerase are adopted to carry out loop-mediated isothermal amplification reaction, so as to detect the ichthyophthirius multifilis. According to the method, visual detection of ichthyophthirius multifilis can be achieved within 20 min, professional knowledge or special fluorescent dye is not needed, and whether a sample contains the ichthyophthirius multifilis or not can be judged only by observing the color of an amplification product. The method can be used for detecting ichthyophthirius multifilis of which the plasmid DNA concentration of a sample is as low as 0.01 ng / mu L, and is high in sensitivity and good in specificity. According to the method, rapid early warning can be achieved in the early outbreak stage of the ichthyophthirius multifilis, early discovery and early prevention are achieved, economic losses are reduced, and the method has wide application prospects.

The application discloses a bacillus megaterium strain for increasing adversity tolerance of aquatic animals and application thereof. The bacillus megaterium strain is named bacillus megaterium SCAU-Ohb1, the strain has been preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO.62119, and the preservation date is December 13, 2021. The bacillus megaterium SCAU-Ohb1 is isolated from fish intestinal tracts and is safe to fish bodies. After the fermentation liquor of the bacillus megaterium SCAU-Ohb1 is mixed with a basic daily ration and is used for feeding freshwater fish, the activity of important antioxidant enzymes in the fish bodies can be significantly improved, the salt tolerance of gill tissues of the freshwater fish under acute salt stress can be increased, and the survival rate of the fish bodies can be improved. The application provides a new method for improving adversity tolerance of aquatic animals, has important significance for the aquaculture industry, and is expected to improve production efficiency and reduce losses.

The invention discloses a running water net cage culture method for coreius fasciatus. The running water net cage culture method comprises the following steps: S1, setting a culture environment and a net cage; s2, fingerling stocking; s3, feeding management; s4, daily management; s5, disease prevention and control; s6, harvesting and transporting, in the step S1, a plurality of net cages are prepared, the net cages need to be placed in a pond or a reservoir with the water depth larger than 4 m, the pond or the reservoir needs good water quality and high dissolved oxygen, every three net cages form a group, a water pushing machine is arranged on one side of one group of net cages, a gas compressor is arranged on the inner side of the water pushing machine, and a baffle is arranged in the water pushing machine. An exclusive breeding mode different from conventional freshwater fishes is provided for coreius guichenoti, the overall design of water flowing and environment regulation is emphasized, and the feeding activity and growth efficiency of coreius guichenoti are improved by improving the breeding environment directly aiming at the core problems of insufficient water exchange, lack of water flow stimulation and the like in still water breeding. And a complete scheme is provided for efficient and healthy coreius coreius culture.

The invention belongs to the technical field of pathogenic microorganism screening and aquaculture disease prevention and control, and discloses an aeromonas salmonicida natural attenuated strain derived from channel catfish and application of the aeromonas salmonicida natural attenuated strain, and the preservation number of the strain is CCTCC NO: M20252718. Through artificial infection, the toxicity of the strain on tested rainbow trout and grass carp is far lower than that of an aeromonas salmonicida virulent strain, and a live vaccine prepared from the strain is used for performing immune protection on rainbow trout and grass carp, so that bacterial diseases caused by the aeromonas salmonicida virulent strain can be effectively prevented. In the culture process, the strain can effectively reduce the use of antibiotic drugs, and has a positive promotion effect on reducing drug residues of freshwater fishes and further realizing green culture.

The invention belongs to the field of animal model construction, and particularly relates to a construction method and application of an early embryonic development cataract eye disease animal model. The invention finds that acute exposure of N-(1, 3-dimethylbutyl)-N '-phenyl-p-phenylenediamine (6PPD) and polystyrene microspheres (PS) in an embryonic development stage can cause eye dysplasia and visual dysfunction in a zebra fish larva stage, and the zebra fish has typical lens opacification-like cataract pathological characteristics; meanwhile, the phenomena of phototactic behavior inhibition, eye cell death increase and the like occur. The 6PPD / PS treatment interferes with visual perception and phototransduction pathways, significantly affects photoreceptor outer segment, ferroptosis, endocytosis, retinoic acid metabolism and other pathways, and can enhance the activation of ferroptosis regulation genes by enhancing oxidative stress and mitochondrial dependent apoptosis of eye tissues, thereby aggravating the cataract process. The invention finds that cataract can be generated through the 6PPD / PS effect in the development process of freshwater fish vertebrates for the first time, and provides model support for research on pathogenesis and intervention strategies of the freshwater fish vertebrates.

The invention provides an establishment method and application of a pseudorasbora parva snout-end cell line, and belongs to the technical field of freshwater fish cell culture.The establishment method comprises the following steps that (1) primary culture is conducted, specifically, pseudorasbora parva snout-end tissue is digested through collagenase II, a cell / tissue suspension is prepared and inoculated into a culture bottle subjected to polylysine coating treatment, and a culture medium is obtained; culturing by using a primary culture medium; (2) subculture: when the cell generations are 1-20 generations, culturing by using a primary culture medium which contains an epidermal growth factor, a chick embryo extract and 20% fetal calf serum; after more than 20 generations, a subculture medium is used for culture, and the subculture medium contains 10% of fetal calf serum and does not contain epidermal growth factors and chick embryo extracts; and (3) collecting passage cells to obtain the pseudorasbora parva snout end cell line. The pseudorasbora parva snout end cell line established by the method can realize continuous passage and proliferation, can be recovered after ultralow temperature cryopreservation, and can be applied to the fields of exogenous gene expression research, water environment pollutant monitoring and the like.

The invention discloses a green field fish fin ray cell line as well as a construction method and application thereof, and belongs to the technical field of freshwater fish cell culture, the green field fish fin ray cell line is named as green field fish fin ray cell line CQF, and is preserved in China Center for Type Culture Collection on April 2, 2025, and the preservation number is CCTCC NO: C202591. The cell line is derived from green field fish, and on the basis of fin rays of the green field fish, the culture method comprises the following steps: selecting the green field fish with excellent health conditions, temporarily rearing at room temperature for 2 weeks, feeding once in the morning and once in the evening, simultaneously preparing a primary culture medium and a subculture medium, treating fin ray tissues, performing primary culture on fin ray tissue cells, performing subculture, and performing cell cryopreservation and recovery. The green field fish fin ray cell line provided by the invention has the advantages of strong division ability, fast growth speed, good stability, strong repeatability, simple and easy operation, and high recovery activity, and can establish a fish hypoxia stress model as a new in vitro research tool, accelerate the breeding process of good varieties, and improve the breeding efficiency.

The application provides a salt-tolerant bacillus for freshwater fish culture, and the preservation number is CGMCC No.32389.The salt-tolerant bacillus CAG2307-32 strain provided by the application has no pathogenicity to crucian carp, can be applied in a large amount in the freshwater fish culture production such as crucian carp, can effectively regulate water quality, promote fish growth, and enhance the oxidation resistance and immune level of fish, and has great application potential.

The invention belongs to the technical field of ecological environmental protection, and discloses a freshwater fish habitat protection effect and priority evaluation method in a protection area, and the method comprises the steps: employing the collinearity of VIF test variables, employing a MaxEnt algorithm to construct a species distribution model of each species in global sea river migration fishes, and simulating the habitat distribution based on a habitat model; according to the performance of the AUC and TSS evaluation model, simulation of the model is realized; according to the distribution information of the protection area, the stability of the sea-river migratory fish habitats outside and inside the protection area and the planning priority of the protection area are evaluated respectively. According to the method, the protection effect of the land protection area on the freshwater fish habitat and the protection area planning priority for the freshwater fish habitat are evaluated respectively, and the unbalance problem that in the prior art, freshwater fishes are often ignored in the protection area setting process is solved.