The invention relates to a method for inversing soil moisture content from AMSR-E (Advanced Microwave Scanning Radiometer-EOS) data, which can be used for the remote sensing application departments of monitoring weather and environment, managing land, monitoring a drought and a crop growing situation, monitoring disasters, and the like. The method comprises the following four steps of: firstly, simulating the most part of situations on the earth surface and establishing a database by using a theoretical model; secondly, establishing an inverse algorithm by using a simulation database; thirdly, carrying out the inverse calculation of passive microwave data AMSR-E; and fourthly, carrying out proper evaluation, analysis and modification by using observation data of a meteorological department, thereby increasing the precision and the applicability of the algorithm. The method for inversing soil moisture content from the AMSR-E data can be used for the departments of forecasting weather, monitoring environment, agricultural situations and disaster situations, and the like. The microwave radiation frequency arrangement carried by a Fengyun 3 satellite of China is basically similar to the AMSR-E, and the invention can be applied to the Fengyun 3 satellite of China and a microwave radiometer transmitted by China in the future after being modified.