The invention discloses dynamic calibration equipment for a six-dimensional force sensor and a loading method of the dynamic calibration equipment, and belongs to the technical field of sensor testing. The dynamic calibration equipment comprises a lower bed body, a to-be-tested sensor, a connecting plate, a displacement sensor, an electric cylinder, a speed reducer, columns, a bearing plate, a servo motor, a connector, an upper bed body, a standard sensor, a servo motor, a working plate, a connector, a standard sensor, an electric cylinder, a fixing plate, a vertical plate, a loading column, achuck and a PLC control box. According to the dynamic calibration equipment, based on the basic principle of a negative step load, through the rotation of the servo motor, a load is slowly applied tothe to-be-tested sensor, the force value is stabilized by relying the self-locking performance of the electromagnetic brake of the servo motor, the servo motor is controlled by a PLC to rotate reversely, and instantaneous unloading can be conducted to form the negative step load. The dynamic calibration equipment can calibrate the FX, FY, FZ, MZ, MX and MY of the six-dimensional force sensor witha large force value, is small in occupied space, precise and reliable, and can simultaneously meet the requirements of dynamic calibration, static calibration and quasi-static calibration.