The invention discloses a piston for a disposable sterile syringe. The piston is prepared from the following raw materials in parts by weight: 1.0-2.5 parts of activated zinc oxide, 0.5-1.5 parts of stearic acid, 1.0-2.0 parts of environment-friendly anti-aging agent 1010, 10-15 parts of carbon black N330, 50-60 parts of calcined clay, 1.5-3.0 parts of petroleum jelly for medical purposes, 0.2-0.6 part of accelerant TMTM, 0.2-0.5 part of accelerant TRA, 1.0-2.0 parts of sulfur and 100 parts of isoprene synthetic rubber. The manufacturing method comprises the following steps: taking the raw materials as ingredients, mixing with the isoprene synthetic rubber, respectively manufacturing rubber sheets, manufacturing a semi-finished product, removing flashes, cleaning, drying and checking. The piston for the disposable sterile syringe, which is made of the isoprene synthetic rubber, is excellent in biological performance index, good in ageing resistance, high in resilience force and excellent in self-sealing performance, a pyrogen reaction is avoided, lots of energy is saved, and the environmental pollution is reduced.