The invention provides an antimicrobial UV curing printing ink, comprising 7-10wt% of compound antibacterial agent, 10-25wt% of pigment, 33-62wt% of UV vehicle, 3.9-9wt% of photoinitiator, 4-40wt% of diluent, and 1-4wt% of auxiliary agent. The invention further provides a preparation method of the antimicrobial UV curing printing ink. According to the invention, the problems of no antibacterial effect, impact on people' health, unsanitariness, etc. of UV printing inks in the prior art are overcome, and the antimicrobial UV curing printing ink disclosed herein has high-efficiency antibacterial property, good usage effect, and wide range of application, can effectively cut off the propagation approach of pathogens and guarantee people' health, and has good market prospect, etc.