The invention discloses nature bamboo fiber kitchen towels with high antibacterial property and a preparation method. The preparation method comprises the following steps: S1, washing bamboo sheets, removing impurities of the bamboo sheets and performing dehydration for subsequent use; S2, steaming and cooking the bamboo sheets by adopting an alkali method; S3, screening out bamboo joints and coarse fiber bundles of pulps which are obtained after steaming and cooking, and processing the bamboo joints and the coarse fiber bundles by adopting a hot water counter-current washing method; S4, concentrating the obtained nature bamboo pulp fibers through a press filter, and performing high-concentration pulp grinding and low-concentration pulp grinding to obtain paper pulps; S5, performing dilution, sand removal, molding, dehydration, drying, wrinkling and rolling on the paper pulps to obtain raw paper; and S6, slicing and packaging the raw paper. The nature bamboo fiber kitchen towels with the high antibacterial property disclosed by the invention adopts a sulfur-free steaming and cooking process, an oxygen delignification step which is adopted generally in the industries is eliminated,and chloride-containing bleaching treatment is avoided, and therefore, a production process is green and environmentally friendly, the properties of bamboo raw materials are kept to the maximum extent, the nature bamboo fiber kitchen towels have a faint fragrance of bamboos, and bamboo quinone contained in the pulp has a more natural antibacterial effect.