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31results about How to "Overcoming Technical Barriers" patented technology

Preparation method of fresh-wet noodles

The invention discloses a preparation method of fresh-wet noodles, comprising the following steps: A1, pretreating flour: selecting flour without addictive, regulating protein content, and regulating water absorption; and A2, manufacturing noodles: kneading dough, proofing, tableting, slitting, weighting, packaging and refrigerating, wherein in the step of kneading dough, water amount is calculated according to the type of knead dough equipment, the total water is prepared into antibiosis refreshment aqueous solution with 2% of propanediol and 2% of alcohol, the antibiosis refreshment aqueoussolution is divided into two parts, a flour modifying agent I is dissolved by using 10-fold antibiosis refreshment aqueous solution of 60 DEG C, and a modifying agent II is dissolved by using the rest of antibiosis refreshment aqueous solution. The noodles produced by the method are stable in quality, are long in storage period (namely, preservation for 10-15 days at the low temperature of 3-8 DEG C), have the characteristics of no muddy soup, refresh mouth feel, vein wires, elasticity, smooth surface, no sticky, soak-resistance and no crisp, thereby achieving the cooking requirements of high-grade noodles with soup.
Owner:QISHAN BAINIANMEIYANG CUSTOM FOOD

System architected by SOA-based application system platform and design method

The invention discloses a system architected by an SOA (Service-Oriented Architecture)-based application system platform and a design method. By designing a base layer, a platform layer, an interface layer, an application service layer, a subnet interface layer and a subnet layer in the SOA application system platform, and by the platform to unify an outward interface specification, and to interconnect outward interfaces with external systems, a new SOA-based application system platform architecture, which is in line with SOA architecture principle, is loose in coupling, is coarse in granularity, has strong adaptability for changes in a user system and is completely different from an ESB / EMB platform, is realized. The SOA-based application system platform architecture is suitable for architecting of medium and small scale system platforms, and is particularly suitable for an Information-Island type system with relatively concentrated business. The system and the method help to solve the problems that the ESB / EMB platform structure is too loose, the input cost is high, the architecting period is long, and different manufacturers have difficulty in collaboration.
Owner:CHINA DIGITAL VIDEO BEIJING

High-efficient inorganic flame retardant and preparation method and application thereof

The invention discloses high-efficient inorganic flame retardant and a preparation method and application thereof, relates to the high-efficient inorganic flame retardant and the preparation method and application thereof, and aims at solving the problem that the mechanical performance of a material is deteriorated by poor flame retardant efficiency and higher loading of existing metal hydroxide flame retardant. The flame retardant is prepared from, by weight, 100 parts of inorganic flame retardant and 0.01-20 parts of polycarbosilane. The method comprises the steps that step one, polycarbosilane solution is prepared and mixing is conducted; step two, the polycarbosilane and the inorganic flame retardant are put into a high-speed mixer and mixed; step three, the polycarbosilane and the inorganic flame retardant are put into a ball grinding mill and milled. The inorganic flame retardant is used for preparing a halogen-free flame-retardant polyolefin material. The oxygen index (OI) of the prepared halogen-free flame-retardant polyolefin material is 25-40, the tensile strength is 10 MPa-20 MPa, and the elongation is 180%-450%. By means of the method, the high-efficient inorganic flameretardant can be obtained.
Owner:HARBIN UNIV OF SCI & TECH

Organosilicon vinyl acetate-acrylic emulsion and preparation method thereof and redispersible emulsion powder prepared from organosilicon vinyl acetate-acrylic emulsion

The invention discloses organosilicon vinyl acetate-acrylic emulsion and a preparation method thereof and redispersible emulsion powder prepared from the organosilicon vinyl acetate-acrylic emulsion. The emulsion is prepared from, by mass, 60-80 parts of vinyl acetate, 12-30 parts of butyl acrylate, 5-15 parts of a functional monomer, 1-6 parts of a crosslinking monomer, 0.2-3 parts of a silane coupling agent, 3-10 parts of protective colloid, 0.2-5 parts of an emulsifying agent, 0.2-0.6 part of a buffering agent, 0.2-0.6 part of a redox initiator and 90-120 parts of water. According to the organosilicon vinyl acetate-acrylic emulsion, by adopting a pre-emulsification method, the emulsion stability is greatly improved, the gelation phenomenon caused by instability of the emulsion in the polymerization process is effectively reduced, and the emulsion preparation success rate is increased; by introducing the crosslinking monomer, the functional monomer and the silane coupling agent into macromolecular chains of a polymer, crosslinking points on the polymer are increased, and a dense three-dimensional net is formed during the film forming process, so that the water resistance is improved, and then the application property of the redispersible emulsion powder in the application fields of building mortar, building waterproof paint and the like is promoted.
Owner:YOUNGWOO CHEMTECH

Outdoor billboard

The invention provides an outdoor billboard. The outdoor billboard is connected with a bus system and is characterized by comprising a plurality of electronic billboard bodies and a computer center, wherein the electronic billboard bodies are used for playing bus information; the computer center is connected with the electronic billboard bodies and the bus system respectively and is used for receiving and processing query information; each electronic billboard body is provided with a display module, an audio input module, a voice recognition module, a billboard terminal communication module, inquiry button keys and a host, wherein the display module is used for playing the bus information, the audio input module is used for receiving user query information, the voice recognition module is used for recognizing the query information as recognized information, the billboard terminal communication module is connected with the computer center and issuing the recognized information, the inquiry button keys are arranged at the outer side of the corresponding electronic billboard body so as to prevent misoperation, and the host is connected with the display module, the audio input module, the voice recognition module, the billboard terminal communication module and the inquiry button keys respectively; the computer center is provided with a central terminal communication module which is connected with the billboard terminal communication modules and the bus system respectively.
Owner:SHANGHAI PUBLISHING & PRINTING COLLEGE

Preparation method of printed circuit board and copper foil for processing

The invention provides a preparation method of a printed circuit board. The method includes the following steps of: S1, copper foil arrangement: copper foil is arranged on a carrier in a manner that the copper foil is aligned with the carrier, and the copper foil and the carrier are composited; S2, laser cutting: laser cutting is performed on the pattern of the circuit diagram of a circuit board;S3, selective peeling: an aluminum substrate is coated with photoresist, and a mask with a circuit diagram is arranged on the aluminum substrate, and the aluminum substrate is selectively exposed according to the pattern of the circuit diagram, so that the non-circuit portion of the circuit diagram loses viscosity; and S4, printed circuit board preparation: the copper foil arranged on the carrieris aligned with the aluminum substrate, so that the circuit diagrams of the copper foil and the aluminum substrate are completely overlapped with each other, and the copper foil of a circuit portion is completely bonded to the aluminum substrate, and the copper foil of a non-circuit portion is peeled off. According to the preparation method of the invention, the circuit board is inscribed by a laser cutting method, so that chemical etching can be avoided in a production process; and waste copper can be directly recycled, and therefore, a great environmental protection value can be realized with cost reduced. With the method adopted, a new idea is provided for the production of copper foil for circuit board.
Owner:北京启创驿讯科技有限公司

A kind of synthetic method of bisfluorosulfonyl imide lithium

The invention provides a method for synthesizing lithium bis(fluorosulfonyl)imide. The synthetic method of the lithium bis(fluorosulfonyl)imide is characterized by comprising the following steps: (1) in a nitrogen atmosphere, sufficiently mixing bis(fluorosulfonyl)imide and an organic solvent, and putting a mixture into a reactor with a condenser; sequentially adding lithium fluoride and liquid-state hydrogen fluoride into the reactor and raising the temperature to react; (2) reducing the temperature of a reaction system, and decompressing and displacing by nitrogen to completely remove residual hydrogen fluoride gas, and filtering a reaction solution under the nitrogen atmosphere to remove insoluble matters, so as to obtain filtrate; (3) concentrating the filtrate by utilizing a high-vacuum decompression and desolvation method; (4) cooling a concentrated solution and adding a low-polarity solvent; recrystallizing at low temperature, and filtering and carrying out decompression and drying treatment at 40 DEG C to obtain the pure lithium bis(fluorosulfonyl)imide. The synthetic method has the advantages that used reagents are cheap and easy to obtain, a preparation process is simple, a reaction is rapid and complete, a few of byproducts are produced and are easy to remove, a post-treatment manner is simple and convenient, a product has high purity and the synthetic method is very suitable for industrial production.
Owner:RONGCHENG QINGMU CHEM MATERIALS

Electrostatic chuck device

The invention discloses an electrostatic chuck device and belongs to the technical field of semiconductor wafer processing. The electrostatic chuck comprises a first insulation layer, a second insulation layer, an electrode layer and a metal layer. The first insulation layer is arranged above the second insulation layer. The electrode layer is arranged between the first insulation layer and the second insulation layer. The second insulation layer is arranged on the metal layer. The first insulation layer is of sapphire material. The electrostatic chuck device has strong anti-corrosion capability and uniform electrostatic absorption capacity, and has low requirements for equipment.
Owner:BEIJING U PRECISION TECH

AC-DC chip and high-voltage free-wheel diode integrated chip structure and power module group

The invention provides an AC-DC chip and high-voltage free-wheel diode integrated chip structure, and belongs to the technical field of electronic device. The structure is characterized in that the interior of a plastic package body is provided with a first chip, a second chip, at least one lead frame, and a plurality of metal leads. The first chip is an AC-DC chip, and the second chip is a free-wheel diode chip. One bonding pad of the first chip is connected with a high-voltage power supply pin of the plastic package body through the metal leads, and a drain electrode of the first chip is connected with an anode of the second chip, wherein the surface of the cathode of the second chip is provided with a bonding pad, and is connected with the high-voltage power supply pin. The anode of thesecond chip is connected with a lead frame, and is lead out of the plastic package body to serve as a drain electrode pin. The invention also discloses the power module group employing the above integrated chip. Two core devices in a power drive board are integrated into one chip, and the number of pins of the new integrated chip and the pin positions still can be completely compatible with an original AC-DC chip, thereby avoiding the increase of the size of the chip while reducing the number of drive power devices, improving the production efficiency, and reducing the size and cost of the power supply.
Owner:SHANDONG JINGDAO MICROELECTRONICS +1

Fouling prevention method and method for olefin oligomerization

The present invention provides a fouling prevention method and a method for olefin oligomerization, wherein in the method for olefin oligomerization, a predetermined anti-fouling agent is added, thereby minimizing the production of sticking byproducts generated during the reaction and fundamentally preventing the fouling of the byproducts, generated during the reaction, on an inner wall of a reactor.
Owner:SK INNOVATION CO LTD +1

Pumping liquid asphalt loading system and process

The invention relates to a pumping liquid asphalt loading system and process. The system comprises a loading system and a maintenance and cleaning system. The loading system comprises an asphalt storage tank, an asphalt loading pump, an automobile loading arm and an automobile tank car. The maintaining and cleaning system comprises an anthracene oil tank and an underground emptying tank. The process comprises the following steps of 1) carrying out loading preparation; 2) loading asphalt; 3) purging and moving the vehicle; and 4) maintaining and cleaning. The maintenance and cleaning system isadditionally arranged on the basis of the asphalt loading system, a technical barrier restricting an application of a liquid asphalt pumping and loading process is overcome, and the process has high practicability, is suitable for intermittent operation of liquid asphalt loading and is good for application and popularization. An operation procedure is simple and convenient, and whole-process automatic operation can be achieved.
Owner:ACRE COKING & REFRACTORY ENG CONSULTING CORP DALIAN MCC

Movable outdoor breeding facility

The invention provides a movable outdoor breeding facility and relates to the technical field of livestock breeding. The movable outdoor breeding facility comprises main rods, auxiliary rods and baffles. Each main rod comprises a vertical rod, a cross chassis and an inclined strut, wherein the vertical rod is arranged in the center of the cross chassis, the inclined strut is arranged between the vertical rod and the cross chassis, and a right-angle groove and a bolt hole are formed in the top of the vertical rod. Each baffle is formed by connecting concave ends at the two ends and a middle plate, the concave ends are clamped on the vertical rods, spacer blocks are arranged between the baffles, pressing rods are installed in the right-angle grooves in the top ends of the vertical rods, glands are installed on the tops of the vertical rods, and the glands are installed above the pressing rods and fastened to the tops of the vertical rods through bolts. Planting and breeding are combined, green breeding is achieved, pigs are bred among forests (between line spacing), and poultry is bred among forests (between row spacing); waste land and uncultivated land are improved; land crop rotation for bringrecovery is achieved, land phytotoxin and microorganism harm is retarded, and pesticide and chemical fertilizer are reduced.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI

Screening method of single memory B cell and application of single memory B cell in preparation of small-molecular monoclonal antibody

The invention provides a screening method of a single memory B cell and application of the single memory B cell in preparation of a small-molecular monoclonal antibody. The screening method is based on a fluorescence-activated cell sorting technology and a microfluidic technology, organic combination of accurate screening of an antigen-specific memory B cell and efficient preparation of a single cell transcript in a microenvironment is realized, and single cell variable region pre-amplification, scFv prokaryotic expression and eukaryotic expression of the monoclonal antibody are further carried out. A novel single cell antibody preparation technology is established, and a new means is provided for preparation of the small-molecular rabbit monoclonal antibody.
Owner:CHINA AGRI UNIV

A printed circuit board processing method, system and copper foil for processing

The present invention provides a printed circuit board processing method, which includes the following steps: S1, laser cutting: using laser to cut out a circuit needed on the copper foil which is pasted on the carrier; S2, preferential viscosity processing: carrying out preferential viscosity processing to the copper foil and the carrier; S3, preferential adsorption: carrying out preferential adsorption to the cut copper foil corresponding to the non-circuit part; S4, peeling: peeling the non-circuit part of copper foil off the carrier to obtain the copper foil corresponding to the circuit part attached to the carrier; S5, preparation of the printed circuit board, transferring the copper foil of the circuit part on the carrier to the baseboard to obtain the printed circuit board. It is possible to use laser cutting to cut the printed circuit board, avoid chemical milling in the manufacture process, and directly recycle the waste copper, is of great value of environmental protection while reducing the cost, and a new thought for preparing printed circuit board with copper foil is provided.
Owner:曹昕

Preparation method of fresh-wet noodles

The invention discloses a preparation method of fresh-wet noodles, comprising the following steps: A1, pretreating flour: selecting flour without addictive, regulating protein content, and regulating water absorption; and A2, manufacturing noodles: kneading dough, proofing, tableting, slitting, weighting, packaging and refrigerating, wherein in the step of kneading dough, water amount is calculated according to the type of knead dough equipment, the total water is prepared into antibiosis refreshment aqueous solution with 2% of propanediol and 2% of alcohol, the antibiosis refreshment aqueous solution is divided into two parts, a flour modifying agent I is dissolved by using 10-fold antibiosis refreshment aqueous solution of 60 DEG C, and a modifying agent II is dissolved by using the rest of antibiosis refreshment aqueous solution. The noodles produced by the method are stable in quality, are long in storage period (namely, preservation for 10-15 days at the low temperature of 3-8 DEG C), have the characteristics of no muddy soup, refresh mouth feel, vein wires, elasticity, smooth surface, no sticky, soak-resistance and no crisp, thereby achieving the cooking requirements of high-grade noodles with soup.
Owner:QISHAN BAINIANMEIYANG CUSTOM FOOD

Method for implementing real name electric commerce, electric government affairs card

InactiveCN101350085BReduce original investmentImprove anti-counterfeiting performanceCommerceMemory systemsPasswordUniversal Credit
The invention relates to a method for realizing a universal credit card of real-name system electronic commerces and electronic governments, which is applied in various real-name system electronic commerce and electronic government activities. The method is characterized in that a serial number which is in a radio frequency IC card (RFID) and is inherent in a production factory and a cardholder ID card number which is stored are adopted to combine into a registered number which can be recognized and used by various electronic commerce systems and electronic government systems together. A method for distinguishing the false and authenticating of the invention comprises: storing a name, a mobile phone number and a manual password of a card holder into a registration number domain of the electronic commerce systems when a card is sent and licensed and adopting: firstly, a combination attestation mode of the manual password and the registered number in the IC card, secondly, the combination attestation mode of the mobile phone number and the manual password, thirdly, the combination attestation mode of the registered number in the IC card, the manual password and the mobile phone number when electronic commerce activities are carried out. Double mobile and network channels are adopted to finish the electronic commerce and electronic government activities to realize the universal credit card of various electronic commerces and electronic governments.
Owner:王智强

A kind of preparation method of printed circuit board and copper foil for processing

The invention provides a preparation method of a printed circuit board. The method includes the following steps of: S1, copper foil arrangement: copper foil is arranged on a carrier in a manner that the copper foil is aligned with the carrier, and the copper foil and the carrier are composited; S2, laser cutting: laser cutting is performed on the pattern of the circuit diagram of a circuit board;S3, selective peeling: an aluminum substrate is coated with photoresist, and a mask with a circuit diagram is arranged on the aluminum substrate, and the aluminum substrate is selectively exposed according to the pattern of the circuit diagram, so that the non-circuit portion of the circuit diagram loses viscosity; and S4, printed circuit board preparation: the copper foil arranged on the carrieris aligned with the aluminum substrate, so that the circuit diagrams of the copper foil and the aluminum substrate are completely overlapped with each other, and the copper foil of a circuit portion is completely bonded to the aluminum substrate, and the copper foil of a non-circuit portion is peeled off. According to the preparation method of the invention, the circuit board is inscribed by a laser cutting method, so that chemical etching can be avoided in a production process; and waste copper can be directly recycled, and therefore, a great environmental protection value can be realized with cost reduced. With the method adopted, a new idea is provided for the production of copper foil for circuit board.
Owner:曹昕

High-survival-rate crayfish culture method in high-temperature season

The invention discloses a high-survival-rate crayfish culture method in a high-temperature season, and belongs to the technical field of crayfish culture. The high-survival-rate crayfish culture method in the high-temperature season comprises the following steps of 1) preparing a pond; 2) transporting and putting young shrimps; 3) culturing young shrimps; and 4) separating the young shrimps and culturing adult shrimps. According to the high-survival-rate crayfish culture method in the high-temperature season, on one hand, the crayfish seeds can be effectively prevented from death in the transportation process by adopting a gradient cooling pure oxygen filling packaging transportation mode, and on the other hand, culturing and seed separating are carried out on the crayfish seeds by utilizing a temporary culture pond, so that the situation that large-batch death occurs when the crayfish seeds are put in the high-temperature season is avoided, centralized management of the crayfish seeds is facilitated, and the survival rate of adult shrimp culture is greatly improved.
Owner:武汉正大水产有限公司

Method for producing high-purity donepezil hydrochloride anhydrous I type crystal form and product thereof

The invention relates to a method for producing a high-purity donepezil hydrochloride anhydrous I type crystal form and a product thereof, belonging to the technical field of drug synthesis. The method comprises the following steps of: re-crystallizing a donepezil hydrochloride polycrystal type crude product or an amorphous donepezil hydrochloride crude product with a lower alcohol solvent to obtain a donepezil hydrochloride I type crystal form wet product; and drying in vacuum and cooling to the room temperature to obtain a high-purity donepezil hydrochloride anhydrous I type crystal form finished product under the protection of nitrogen, wherein KF is less than 0.5%; the purity reaches more than 99.5%; and single impurity is less than 0.1%. The invention has the advantages of simple production method and fewer steps, is suitable for industrial production, has better crystal form of the product and great significance to pharmacy, sale and storage of the product, and overcomes technology barriers.
Owner:浙江东亚药业股份有限公司

Flexible conductive adhesive horizontal vibration conveying device

The invention discloses a flexible conductive adhesive horizontal vibration conveying device which comprises a linear conveying track and a vibrator installed on the linear conveying track. The upperplane of the linear conveying track is provided with a groove communicating from one end of the linear conveying track to the other end; the bottom of the groove is provided with a slide communicatingfrom one end of the groove to the other end; a conductive adhesive limiting baffle used for limiting conductive adhesives conveyed by the slide from vibrating out of the slide is arranged over the slide; and when the vibrator vibrates the linear conveying track, as the flexible conductive adhesives are oval, the conductive adhesives vibrate on the slide without being overlapped and clamped, and the multiple conductive adhesives on the slide are vibrated, arranged and conveyed to an outlet of the slide one by one in sequence. The flexible conductive adhesives are shaped and positioned throughthe mechanical and electric control combined method, the technical problem of difficult positioning of the conductive adhesives is solved, the method is accurate and rapid in positioning, and the structure is simple. The flexible conductive adhesive horizontal vibration conveying device is only suitable for vibration conveying of the flexible conductive adhesives in specific shapes.
Owner:东莞市本润机器人科技股份有限公司

AR scene design system

ActiveCN110675503AOvercoming the defects of passive operationImplement analog trackingStands/trestlesImage data processingSimulationDisplay device
The invention relates to an AR scene design system. Four limiting support columns are fixedly mounted at four corners of a scene movement simulator mounting port; the tops of the four limiting supportcolumns are connected with the bottom of the mobile simulation limiting ring; a scene movement simulation device is mounted in the scene movement simulator mounting opening below the movement simulation limiting ring; a controller is mounted in the bottom supporting box body on one side of the scene movement simulation device; a displacement catcher is fixedly mounted at the bottom of the top scene protection plate above the moving simulation limiting ring; the displacement capturer, the scene displayer and the scene movement simulation device are connected with the controller through wires,the controller is connected with an external computer through an external input and output line, and a user can obtain more real experience compared with traditional augmented display equipment.
Owner:HUNAN UNIV OF ARTS & SCI

Plate magnetic pulse and gas expansion superplastic composite forming device and method

The invention discloses a plate magnetic pulse and gas expansion superplastic composite forming device and method, and relates to the field of metal material machining and forming. The problems that in the prior art, an electric pulse and gas expansion combined mode is low in forming speed and low in precision of formed parts are solved. The plate magnetic pulse and gas expansion superplastic composite forming device comprises a mold unit, a gas expansion unit, a heating unit and a magnetic pulse unit; the heating unit is used for heating a plate to be processed; the mold unit is used for fixing a to-be-processed plate; the magnetic pulse unit is used for performing multiple times of pulse excitation on the to-be-processed plate, so that the plate is preformed for multiple times; and the gas expansion unit is used for introducing high-pressure gas into the mold unit and carrying out multiple times of gas expansion stamping forming on the preformed plate, so that processing of the plate is completed. The plate magnetic pulse and gas expansion superplastic composite forming device is mainly used for processing and forming plates.
Owner:INNER MONGOLIA UNIV OF TECH

Screen printing plate with high adhesion and manufacturing process thereof

The invention discloses a high-adhesion screen printing plate and a manufacturing process thereof. The high-adhesion screen printing plate comprises an annular frame body, an annular connecting net, silk screen gauze, an annular adhesion layer and a high-molecular carving film. According to the process disclosed by the invention, a traditional operation mode of firstly carrying out hot pressing on a hot melting film and then carrying out hot pressing on a PI film is changed, and the process disclosed by the invention is an operation mode of firstly carrying out hot pressing on the PI film and then carrying out hot pressing on the hot melting film; then the macromolecular carving film and the silk-screen mesh are placed on the connecting net made of polyester after being subjected to hot pressing, and then the connecting net and the silk-screen mesh are subjected to hot pressing through the annular bonding layer made of the hot-melt film material, so that the annular bonding layer made of the hot-melt film material subjected to hot pressing cannot be melted and drop by high temperature; and the area of the high-molecular carving film made of the PI film material, which is hot-pressed at the upper end of the silk-screen gauze, is larger.
Owner:JIANGSU SHENGXI ELECTRONICS SCI & TECH

A kind of inorganic flame retardant and preparation method and application

The invention discloses high-efficient inorganic flame retardant and a preparation method and application thereof, relates to the high-efficient inorganic flame retardant and the preparation method and application thereof, and aims at solving the problem that the mechanical performance of a material is deteriorated by poor flame retardant efficiency and higher loading of existing metal hydroxide flame retardant. The flame retardant is prepared from, by weight, 100 parts of inorganic flame retardant and 0.01-20 parts of polycarbosilane. The method comprises the steps that step one, polycarbosilane solution is prepared and mixing is conducted; step two, the polycarbosilane and the inorganic flame retardant are put into a high-speed mixer and mixed; step three, the polycarbosilane and the inorganic flame retardant are put into a ball grinding mill and milled. The inorganic flame retardant is used for preparing a halogen-free flame-retardant polyolefin material. The oxygen index (OI) of the prepared halogen-free flame-retardant polyolefin material is 25-40, the tensile strength is 10 MPa-20 MPa, and the elongation is 180%-450%. By means of the method, the high-efficient inorganic flameretardant can be obtained.
Owner:HARBIN UNIV OF SCI & TECH

Quantitative qPCR (quantitative polymerase chain reaction) detection method and kit for tRNA (transfer ribonucleic acid) aminoacylation level

The invention relates to a quantitative qPCR (quantitative polymerase chain reaction) detection method and kit for the aminoacylation level of tRNA (transfer ribonucleic acid), which comprises the following steps: (1) extracting total RNA (ribonucleic acid) without removing amino acid from the tRNA; (2) removing amino acid from tRNA in the total RNA to obtain total RNA containing all deacylated tRNA; according to the present invention, the amino acid sequence and the amino acid sequence are respectively connected with the same tRNA linker, qPCR detection is performed after reverse transcription, the difference value of the Ct value indicates the amino acylation level of the tRNA, and the amino acid load level of the tRNA can be calculated through internal reference gene correction and data standardization processing. Besides, the invention innovatively provides a simple co-reverse transcription method of all tRNA and mRNA for transcribing cell nucleus and mitochondria, the technical barrier of tRNA aminoacylation detection is overcome, and quantitative detection of the tRNA aminoacylation level can be realized by using a conventional qPCR technology.
Owner:SICHUAN AGRI UNIV

A VR social system and method based on real-time human body three-dimensional reconstruction

The invention discloses a VR social system and method based on real-time three-dimensional reconstruction of human body, relates to the technical field of three-dimensional reconstruction technology and virtual reality social realization, and solves the problem caused by the user wearing a head-mounted display that blocks the face in the prior art VR social activities Technical problems such as the inability to generate face point clouds in real time during 3D reconstruction of the human body. The invention not only allows each social object to freely control its position in the virtual scene through human body positioning, but also allows each social object to freely control the angle of view it sees in the head display through poses, and can also use real-time human body three-dimensional Rebuild sees itself and other social objects. The present invention is used in fields such as VR social interaction, meetings, games, and medical research.
Owner:UNIV OF ELECTRONICS SCI & TECH OF CHINA
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