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41results about How to "Shorten mashing time" patented technology

Recombinant high-temperature pullulanase and preparation method thereof

The invention relates to a recombinant high-temperature pullulanase and a preparation method thereof, belonging to the field of bioengineering technology. The invention is characterized in that a gene coding the high-temperature pullulanase is loaded on a pHsh expression vector and is then transferred to an Escherichia coli host for enlarged culture of an engineering bacterium containing the high-temperature pullulanase, then ultrasonic fragmentation and heating are carried out to remove most impurity proteins autonomously expressed by Escherichia coli, then the high-temperature pullulanase is prepared through affinity chromatographic separation and purification, and the recombinant high-temperature pullulanase has an optimal reaction temperature as high as 70 DEG C and a wide pH value application range and performs good catalytic activity at a high temperature of 70 to 80 DEG C, which enables the pullulanase to be especially suitable for cooperative usage with amylase and glucoamylase in the industry of production of sugar from starch and allows saccharification time to be reduced and saccharification efficiency to be improved. The recombinant high-temperature pullulanase provided by the invention is widely applicable to industries like food, wine brewing, feeds, medicine, papermaking and weaving.
Owner:YANGTZE UNIVERSITY

High-efficiency mashing device for cherokee rose fruits used as traditional Chinese medicine

The invention relates to a high-efficiency mashing device for cherokee rose fruits used as traditional Chinese medicine. The high-efficiency mashing device comprises a mashing box body, a crushing box and a controller. The crushing box is fixedly arranged above the mashing box body through supporting columns in a supported manner, and a connection sleeve pipe is fixedly arranged between the mashing box body and the crushing box. The crushing box is internally provided with a crushing storehouse, the top of the crushing box is provided with a material feeding hopper in a welded manner, one side of the crushing box is provided with a crushing motor through bolts, and the output end of the crushing motor stretches into the crushing storehouse and is detachably provided with a crushing tray. The mashing box body is internally provided with an installation storehouse which is internally provided with mashing inner barrels, and the gaps between the mashing inner barrels and the installation storehouse are evenly provided with shock-reducing rubber pads. According to the high-efficiency mashing device, the crushing box is arranged above the mashing box body, so that the cherokee rose fruits pass through the crushing box before being mashed, and the cherokee rose fruits are crushed before being mashed, the mashing time is greatly shortened, the mashing efficiency is improved, the mashing is more thoroughly and the mashing effect is improved.
Owner:CHANGZHOU INST OF DALIAN UNIV OF TECH

Trichoderma reesei bacterial strain for expressing saccharifying enzyme

The invention aims at providing a trichoderma reesei bacterial strain for producing saccharifying enzyme. The trichoderma reesei bacterial strain is trichoderma reesei HDGAU-1 (Trichoderma reesei HDGAU-1) with a preservation number of CCTCC (China Center for Type Culture Collection) NO: M2013584. The trichoderma reesei bacterial strain disclosed by the invention can efficiently express the saccharifying enzyme, and fermentation enzyme activity is as high as 4000 U/ml which is improved by 54% in comparison with that before mutation, and protein amount exceeds 2.1 g/L which is improved by about 50% in comparison with that before mutation. According to the invention, production cost of the saccharifying enzyme can be greatly lowered by utilizing the trichoderma reesei bacterial strain to produce the saccharifying enzyme, and wide application of the saccharifying enzyme is facilitated. Moreover, the saccharifying enzyme recombinant and expressed by the trichoderma reesei bacterial strain disclosed by the invention has an optimum acting temperature of 70 DEG C and an optimum acting pH of 5.5, has catalytic efficiency higher than that of the common saccharifying enzyme from aspergillus niger at present, is more high temperature resistant, can effectively shorten saccharifying time and can lower production cost.
Owner:QINGDAO VLAND BIOTECH GRP

Mechanical activation-metal salt reinforcement-combined starchy raw material saccharification method

The invention discloses a mechanical activation-metal salt reinforcement-combined starchy raw material saccharification method. The method comprises 1, raw material treatment: removing impurities in starchy raw materials, drying the starchy raw materials until water content is less than 25%, carrying out crushing and carrying out screening through a sieve of 40-100 meshes, 2, mixing: mixing the treated starchy raw materials and a metal salt according to a weight ratio of 100: 0.5-5 to obtain a uniform mixture, 3, mechanical activation reaction: putting the uniform mixture into a mechanical activation reactor, carrying out treatment at a mechanical activation temperature of 25-60 DEG C for 10-180min and carrying out ball material separation to obtain the activated starchy raw material, 4, slurry preparation: putting the activated starchy raw material into a slurry preparation tank and adding water into the activated starchy raw material at a temperature of 30-50 DEG C along with stirring so that the activated starchy raw material is stirred and dissolved to form starch slurry with a concentration of 25-40%, and 5, saccharification: putting the raw material slurry into a saccharification tank, adding glucoamylase into the saccharification tank and carrying out saccharification to obtain a saccharification liquid.
Owner:GUANGXI UNIV

Fast saccharification method for sweet potatoes

The invention relates to a fast saccharification method for sweet potatoes. The fast saccharification method includes the steps of firstly, sterilizing, to be more specific, cleaning mud on the surface of the sweet potatoes, washing the sweet potatoes with purified water, and spraying Bordeaux mixture diluent with the ratio of copper sulfate to quick lime being 1:(3-5) to the sweet potatoes to sterilize the sweet potatoes; secondly, transplanting an enzyme source, to be more specific, adhering a saccharifying enzyme patch to the tuberous root umbilicus part of each sweet potato; thirdly, performing microwave irradiation, to be more specific, subjecting the sweet potatoes obtained after the second step to microwave irradiation for 0.5-1 minute to activate the saccharifying enzymes; fourthly, performing anaerobic saccharification, to be more specific, transferring the sweet potatoes obtained after the microwave irradiation into a temperature control room, feeding nitrogen into the temperature control room, and subjecting the sweet potatoes to standing in an environment of 25-35 DEG C for 24-48 hours to perform anaerobic saccharification; fifthly, performing low-temperature storage, to be more specific, after the anaerobic saccharification, storing the saccharified sweet potatoes in a low-temperature room of 5-10 DEG C for 5-10 days. The fast saccharification method has the advantages that the microwave irradiation and saccharifying enzyme catalyzing technologies are utilized, the defects that the prior art is low in saccharification efficiency and speed are overcome, the saccharification time of the sweet potatoes is shortened greatly, and the difficulty that the sweet potatoes are prone to rot during saccharification is solved effectively.
Owner:JIESHOU JINGHUA TECH INFORMATION CONSULTING SERVICE CO LTD +1

Production method of orange flavoring wine

The invention discloses a production method of orange flavoring wine and belongs to the technical field of food fermentation engineering. The method includes: using orange peels and fresh potatoes as raw materials, steaming the peeled fresh potatoes and grinding into mashed potatoes, adding the minced orange peels and an appropriate amount of water while stirring, well mixing, inoculating two different mycete fermentation liquid, using various enzymes in the mycete fermentation liquid to allow the mixture to saccharify, and then adding Luzhou-flavor distillers' grains, Maotai-flavor distillers' grains or both the Luzhou-flavor distillers' grains and the Maotai-flavor distillers' grains for fermentation so as to produce the orange flavoring wine. Compared with traditional cooking wine, the orange flavoring wine produced by the method has the advantages that the organic acid content in the flavoring wine is increased evidently, and an evident deodorizing effect is achieved; the orange peels can increase the flavor and taste of the flavoring wine and increase the quality of the flavoring wine; the release of flavonoid substances in the orange peels allows the flavoring wine to have good healthcare functions such as tonifying the spleen and qi, promoting digestion, resisting fatigue and increasing human immunity.
Owner:HUBEI UNIV OF TECH

Rapid pounding machine capable of isolating noise during processing and used for traditional Chinese medicine Chinese fevervine herb pharmacy process

The invention relates to a fast mashing machine for traditional Chinese medicine chicken vine rattan pharmaceutical process that can isolate processing noise, comprising a base, a mashing box and a crushing box, the mashing box is fixed above the base, and the top of the mashing box is installed A crushing box, and a feed hopper is welded at the center of the top of the crushing box, a material guide groove is provided at the bottom of the inside of the crushing box, and the output end of the material guide groove extends to the top of the crushing bin provided inside the crushing box A mashing motor is installed on one side of the mashing box through bolts, and the output end of the mashing motor extends to the interior of the mashing bin, and is fixedly connected with an eccentric wheel, and a mashing ball is sleeved on the eccentric wheel. The present invention arranges glass fiber sound-absorbing cotton in the side wall interlayer of the smashing box to absorb sound and reduce noise, effectively prevent sound transmission, have stable physical properties and strong vibration resistance, and make the smashing box separate from the external independent space, thereby Effectively isolate the noise generated during the mashing process to avoid affecting people's health.
Owner:高卫东

Traditional Chinese medicine composition co-fermented rice wine and preparation method thereof

The invention provides a traditional Chinese medicine composition co-fermented rice wine and the preparation method thereof, sea cucumber enzymatic hydrolysate, radix puerariae, fructus lycii, fructus jujubae, pericarpium citri reticulatae and sticky rice are proportioned, the sea cucumber enzymatic hydrolysate, enzymatic hydrolysate residues and enzymatic hydrolysate decoction are prepared respectively, effective components in sea cucumber, radix puerariae, fructus lycii, fructus jujubae and pericarpium citri reticulatae are fully extracted, and the enzymatic hydrolysate residues, the enzymatic hydrolysate decoctio and glutinous rice are mixed and steamed, compound yeast is added for fermentation, and the sea cucumber enzymolysis liquid and the enzymolysis medicine juice are added in the middle and later stages of fermentation to continue fermentation, so that the prepared rice wine not only contains active micromolecular sea cucumber polypeptide as a beneficial component, but also is rich in flavone and polysaccharide functional components; the rice wine has good anti-oxidation and beautifying effects, is golden yellow in wine body, transparent in wine quality, elegant in fragrance, and rich in pleasant compound fruit fragrance and fresh rice fragrance.
Owner:海南椰岛酒业发展有限公司

Saccharification method of using extruded white sorghum added with mesophilic enzyme as beer auxiliary material

The invention discloses a saccharification method of using extruded white sorghum added with mesophilic enzyme as beer auxiliary material. The method comprises the following steps: smashing the white sorghum, and then sieving with a sieve with 30-40 meshes; adjusting the moisture weight percent of smashed white sorghum to be 29.5-30.5% and uniformly mixing the smashed white sorghum with mesophilic alpha-amylase, wherein the additive amount of the mesophilic alpha-amylase is 8.5-9.5 L / ton; conducting extrusion treatment on the smashed white sorghum, wherein the extrusion treatment is conducted in a single screw extrusion machine; cutting up extruded white sorghum by a cutter, cooling to room temperature, drying under the condition that the temperature is lower than or equal to 50 DEG C to enable the moisture weight percent of the extruded white sorghum to be 13.0-15.0%, smashing, and sieving with the sieve with 30-40 meshes for later use; simultaneously putting the extruded white sorghum and smashed barley malt into a gelatinization container for saccharification treatment. The method reduces the saccharification time of using the white sorghum as the beer brewing auxiliary material, and improves the filterability of wort and yield of wort extract.
Owner:SHANDONG UNIV OF TECH

A high-efficiency crushing device for Rosa roxburghii used as medicine

The invention relates to a high-efficiency mashing device for cherokee rose fruits used as traditional Chinese medicine. The high-efficiency mashing device comprises a mashing box body, a crushing box and a controller. The crushing box is fixedly arranged above the mashing box body through supporting columns in a supported manner, and a connection sleeve pipe is fixedly arranged between the mashing box body and the crushing box. The crushing box is internally provided with a crushing storehouse, the top of the crushing box is provided with a material feeding hopper in a welded manner, one side of the crushing box is provided with a crushing motor through bolts, and the output end of the crushing motor stretches into the crushing storehouse and is detachably provided with a crushing tray. The mashing box body is internally provided with an installation storehouse which is internally provided with mashing inner barrels, and the gaps between the mashing inner barrels and the installation storehouse are evenly provided with shock-reducing rubber pads. According to the high-efficiency mashing device, the crushing box is arranged above the mashing box body, so that the cherokee rose fruits pass through the crushing box before being mashed, and the cherokee rose fruits are crushed before being mashed, the mashing time is greatly shortened, the mashing efficiency is improved, the mashing is more thoroughly and the mashing effect is improved.
Owner:CHANGZHOU INST OF DALIAN UNIV OF TECH

Method for preparing fatty acid-rich feed material by steam explosion combined with solid-state fermentation

The invention discloses a method for preparing a feedstuff enriched in fatty acid through steam explosion in combination with solid-state fermentation. The method comprises the following steps of putting a starchy biomass raw material in a steam blasting machine to explode; collecting an exploded material, controlling the temperature, adding bran, adjusting the water content and the pH value, and adding medium-temperature amylase and diastatic enzyme to carry out solid-state saccharification; inoculating and culturing an activated sticky silk spore yeast strain to form sticky silk spore yeast seed solution, and inoculating the sticky silk spore yeast seed solution in a saccharified solid-state material according to a certain inoculating amount; and drying and crushing a fermented product so as to obtain the feedstuff. According to the method, the biology and the physical processing method are considered comprehensively; the starchy raw material is exploded and saccharified at first and then the exploded and saccharified starchy raw material is subjected to solid-state fermentation by adding the sticky silk spore yeast; the high-quality feedstuff enriched in fatty acid is produced with low cost; and a more economic and effective energy feedstuff production method is provided for developing the animal husbandry industry.
Owner:河南双成生物科技有限公司 +1
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