The invention discloses high-frequency anti-interference manganese zinc ferrite and a preparation method thereof. The high-frequency anti-interference manganese zinc ferrite comprises main components and auxiliary components, wherein the main components include Fe2O3, MnO and ZnO; the auxiliary components include Bi2O3, MoO3, Co2O3, K2CO3, TiO2, P2O5 and Nb2O5. The preparation method comprises the following steps: weighing the components, doping, pressing into blanks, arranging the blanks and sintering the blanks. Through scientific material formula design, sintering process optimization, furnace atmosphere optimization and other measures, the prepared manganese zinc ferrite has the advantages of relatively high magnetic conductivity characteristic, high magnetic core Curie temperature, good temperature rise characteristic, flat temperature curve, low high-temperature power consumption, strong adaptability to the working environment and the like, so that safety requirements of EMC are met.