The invention discloses a manufacturing process of antibacterial bamboo pulp used for high-wet-modulus fibre. The manufacturing process comprises prehydrolysis, cooking, oxygen delignification, vibration frame screen screening, first washing, sand removal, first concentration, bleaching, second washing, thermal basic refining, pressurized screen preliminary sizing, second concentration, sodium hypochlorite bleaching, third washing, acid treatment, fourth washing, sand removal and manufacture, wherein a hydrolysis accessory ingredient is added in a cooker in prehydrolysis. The hydrolysis accessory ingredient is added in the prehydrolysis, so that the reaction temperature of the prehydrolysis and cooking processes is reduced, thus the content fluctuation of alpha-cellulose is small, the quality is stable, the indexes can meet the pulp requirement of high-wet-modulus fibre, and the natural deodorization, antibacterial and ultraviolet resistance functions of the bamboo fiber can be furthest remained.