The invention provides a trans-rubber fiber material. The trans-rubber is selected from one or more of trans-1, 4-polyisoprene, trans-1, 4-polybutadiene, trans-1, 4-butadiene-isoprene copolymerization rubber, trans-1, 4-butadiene-piperylene copolymerization rubber, trans-1, 4-polydiolefin rubber alloy and trans-1, 4-alkadiene homopolymer and/or homopolymer epoxidation or chlorination modified derivatives and derivatives. The diameter of the trans-rubber is in a range of 100nm to 10 microns. The invention further provides a preparation method and applications of the trans-rubber fiber material. The trans-rubber fiber material has the advantages of being fine in diameter, low in weight, large in specific surface area, large in porosity, small in mass-to-volume ratio, capable of maintaining good fiber morphology after being put for a period and free of fiber dissolving and deformation of other fibers in the prior art.