The invention discloses a method for quickly and sensitively detecting a micromolecule
RNA, and belongs to the field of molecular organisms. The method comprises the following steps: (1) extracting, and purifying a to-be-detected micromolecule
RNA; (2) designing a probe, namely marking the probe by using
biotin or
digoxin; (3) liquid-phase hybridization, namely carrying out hybridization on the to-be-detected micromolecule
RNA and the probe in a
buffer solution,
cutting off un-hybridized excessive
single chain by using excision
enzyme and then carrying out
gel electrophoresis; (4) transferring a membrane, namely transferring the hybridized product on the gel after
electrophoresis into a
solid-phase support (such as nylon membrane, and a
cellulose membrane); (5) incubating
antibody, namely carrying out incubation hybridization on the
solid-phase support containing the hybridized product obtained in the step (4) and a
specific antibody; and (6) detecting a
signal. According to the method, 0.005fmol of miRNA can be detected to the
minimal extent, compared with the prior art, the sensitivity is improved by at least 10 times, the detection is rapid in detection speed, and high-through detection and quantitative detection can be achieved.