The invention provides a tussah silk interwoven mat and a weaving process method. The tussah silk interwoven mat is prepared by interweaving 125dtex/2-250dtex/2 tussah silk twisted warp and 107.62dtex/2-168.6dtex/2 combed cotton thrown weft, wherein the weave structure is 2/2 herringbone twill, the warp density is 200-260/10cm, and the weft density is 180-220/10cm. The product has jewelry luster,elegant and luxurious appearance, has good moisture absorption and sweat discharge effects, is cool in summer and warm in winter, and has multiple health-care functions of resisting acarids, preventing mildews and bacteria, nourishing skin, promoting blood circulation, improving sleep quality, not generating static electricity and the like. The linear product of the herringbone twill structure and high fiber twist has protruded surface texture points, clear grains, obvious concave-convex effect, solid, elastic, stiff and elegant effects and no creping. When the mat is used, point contact between the human body skin and the surface of the mat is formed, thus the air permeability and massage-effect comfortability are effectively increased. In the weaving process, fine weft yarn twist is preferable, thus the product has good interweaving performance; and in the post-treatment process, pre-shrinking treatment is adopted, thus the fabric has excellent dimensional stability and has no deformation after being washed.