The invention provides a piece of novel sewing-free hot melt adhesive TPU-PU leather, and a preparation method thereof. The TPU-PU leather comprises release paper, a hot melt adhesive film, a TPU film and a dry-type PU film, wherein the release paper comprises a paper bottom layer, an adhesive layer, a plastic layer, a transition layer, and a release layer; a hot melt adhesive raw material is composed of polyester polyol, diisocyanate, polyether polyol, and a plurality of additives; a TPU raw material is composed of polymer polybasic alcohol, 1,4-cyclohexane-diisocyanate, a chain extender and a plurality of functional additives; a PU raw material is composed of polyester dibasic alcohol, straight chain diol, isocyanate, TDI trimer, dimethyl formamide, butanone, black sand, and a plurality of functional addictives. Through the adoption of the preparation method, innovations are conducted on the raw material components of the release paper, the hot melt adhesive, the TPU film and the PU film; the manufactured sewing-free TPU-PU leather has the technical advantages of firmness in adhesion, durability, environment-friendly materials, no invisible harm to a human body, and the like.