1results about How to "Improve incompatibility" patented technology

The application discloses a method for improving the sensitivity of RPA-CRISPR-Cas12a detection and application thereof in one-tube detection of chikungunya virus, and relates to the technical field of biology.The full-length crRNA is reformed to form a truncated crRNA (Truncate crRNA), the non-key region is accurately shortened, the space-time regulation of Cas protein activity is realized, and the truncated crRNA plays a key role in CRISPR molecular detection (such as an RPA-CRISPR-Cas12a system). The method can significantly improve the specificity of RPA-CRISPR-Cas12a detection, effectively avoid cross-reaction, inhibit non-specific early activation, significantly reduce background signals, and provide an optimization strategy for developing a molecular diagnostic technology with higher sensitivity.