The invention aims at providing a hydro-pneumatic spring comprising a connecting body, a damping valve, an adjustable pad, an oil cylinder, a main piston, an oil cylinder connector, a guide sleeve, a sealing cover, a lower pin plug, an inflation valve, an air chamber sleeve, an air cylinder, a floating piston, a blocking valve, a small piston, a locking connector and a knuckle bearing. The hydro-pneumatic spring can not only meet the requirement for driving on a smooth road surface, but also meet the requirement for the operation stability index of a vehicle under a bumpy road condition, so that the driving comfort and off-road maneuvering capability are effectively improved.