The invention relates to a multi-stage acceleration rotational flow cavitation device which comprises a first-stage shrinkage pipe, an acceleration pipe, a flow mixing plate, a rotational flow acceleration section, a second-stage shrinkage pipe, a nozzle pipe, a natural vibration cavitation cavity and a flow guide body, and the first-stage shrinkage pipe is located above the acceleration pipe and is in threaded connection with the acceleration pipe; the flow mixing plate is mounted at the uppermost end in the accelerating tube, and an inclined through hole is formed in the flow mixing plate; the rotational flow acceleration section is positioned below the flow mixing plate; the second-stage shrinkage pipe is located below the rotational flow acceleration section, and the diameter of each section of the second-stage shrinkage pipe is smaller than that of the first-stage shrinkage pipe; the nozzle pipe is located below the accelerating pipe, and a converging and flushing nozzle is arranged on the nozzle pipe; the flow guide body is installed at the bottom end of the nozzle pipe, and a through hole matched with the confluence flushing nozzle is formed in the flow guide body in the circumferential direction. The device is simple in structure and high in efficiency, the number of cavitation bubbles is increased through mutual impact of fluid, gas-liquid separation is generated through the rotational flow acceleration section, generation of cavitation bubbles is promoted again, different cavitation strengths are generated through the confluence nozzle, and oil and gas well blockage removal is better facilitated.