The invention discloses a Swerianmarin injection, its preparation process and application, wherein the preparing process comprises using Gentianaceae, Scrophulariaceae or Oleaceae plants as raw material, employing conventional phytochemical method for extraction, segregation and purification, thus obtaining swertiamarin, weighing swertiamarin and medicinal adjuvant, charging water for injection, thermal insulating 10-20 minutes at 30-60 deg. C, cooling down, filtering, dispensing the filter liquor 1-48 hours at 0-5 deg. C, filtering, encapsulating, drying in frozen, sterilizing, and packaging.