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33 results about "Chemical methodology" patented technology

Use of restriction enzymes and other chemical methods to decrease non-specific binding in dual bead assays and related bio-discs, methods, and system apparatus for detecting medical targets

Methods for decreasing non-specific bindings of beads in dual bead assays and related optical bio-discs and disc drive systems. Methods include identifying whether a target agent is present in a biological sample and mixing capture beads each having at least one transport probe affixed thereto, reporter beads each having at least one signal probe affixed thereto, and a biological sample. Mixing is performed under binding conditions to permit formation of a dual bead complex if the target agent is present in the sample. The reporter bead and capture bead each are bound to the target agent. Denaturing the target agent and keeping it in the denatured form by use of a specialized hybridization buffer is also provided. A denaturing agent is guanidine isothiocynate. Methods further include isolating the dual bead complex from the mixture to obtain an isolate, exposing the isolate to a capture field on a disc, and detecting the presence of the dual bead complex in the disc to indicate that the target agent is present in the sample. The methods may further include selectively breaking up non-specific binding between capture beads and reporter beads employing a digestion agent. Also employed is a method for selectively breaking up non-specific binding between capture beads and reporter beads using a wash buffer containing a chemical agent. The methods are applied to detecting medical targets.
Owner:NAGAOKA

Chemical method for synthesising silver selenide semiconductor photoelectric film material in situ at room temperature

The invention provides a chemical method for synthesising a silver selenide semiconductor photoelectric film material in situ at a room temperature. The method comprises the following steps of: dissolving elementary-substance Se powder in Na2S aqueous solution to form orange-yellow solution; placing a substrate material sputtered with an elementary-substance silver film on the surface and the solution aforementioned in the same container; and growing the Ag2Se semiconductor photoelectric film material on the surface of the substrate in situ via a short-time reaction in a temperature range from 7 to 35 DEG C. The reactants are low in price, has no need of being further purified, green and environment-friendly, and any surfactant or other chemical additives are not required; room-temperature reaction conditions are moderate, energy consumption is little, and influence on a conducting substrate is avoided; and the chemical method is fast in reaction, convenient in operation, and controllable in process. The chemical method provided by the invention overcomes the defects of dependence on high vacuum, high energy consumption and high production cost, high reactant toxicity, complex film-forming process and the like of the existing preparation process for an Ag2Se semiconductor photoelectric film material, and is beneficial to large-scale production and industrial application.
Owner:XUCHANG UNIV

Chemical method of chemical protein carrier of plant Chinese herb medicine and carrier chemical protein

The invention discloses a chemical method of a chemical protein carrier of plant Chinese herb medicines and a carrier chemical protein. The carrier chemical protein is prepared in such a way that an egg protein absorbs and carries refined substances in plants necessary for human living cells and is prepared into various prescriptions according to human needs. The chemical method obviously improves the utilization rate of the plant Chinese herb medicines, improves the functions of the treatment and the health care of the plant Chinese herb medicines for human bodies, reduces the side effect of the plant Chinese herb medicines, and has quick and good effect and convenient and easy application. The invention is realized by the following scheme: the prepared plant Chinese herb medicines are put in a utensil according to a prescription by a metering standard of 10g/dose, 120-160 uniform eggs without egg shells are put on the plant Chinese herb medicines, water is added to the range which is 1-1.5 centimeters higher than the eggs, the plant Chinese herb medicines are heated to 100 DEG C, the water temperature is kept at the range of 95 DEG C-100 DEG C for 3.5-4 hours, the color of the egg protein is changed into flesh red, then the egg protein has the functions of the plant Chinese herb medicines of the prescription, and the carrier chemical protein is prepared accordingly.
Owner:甄兆玄

Method of biomolecule immobilization on polymers using click-type chemistry

The present invention provides a method for the covalent immobilization of biomolecules on polymers for delivery of the biomolecules, which has the advantage of being simple, highly efficient, environmentally friendly and free of side products relative to traditional immobilization techniques. The invention provides a modified micro / nanoparticle system, which uses a functionalized polymer formed into micro or nanoparticles to bind a molecule to the particles using uses facile chemistry, the Diels-Alder cycloaddition between a diene and a dienophile with the polymer being functionalized with one of them and the molecule with the other, or the Huisgen 1,3-dipolar cycloaddition between a terminal alkyne and an azide to bind the molecule to the particle. The molecules and / or other therapeutic agents may be encapsulated within the polymer particles for intravenous therapeutic delivery. The invention also provides a novel synthetic biodegradable polymer, a furan / alkyne-functionalized poly(trimethylene carbonate) (PTMC)-based polymer, whose composition can be designed to meet the defined physical and chemical property requirements. In one example, the particle system self-aggregates from functionalized PTMC-based copolymers containing poly(ethylene glycol) (PEG) segments. The composition of the copolymers can be designed to meet various particle system requirements, including size, thermodynamic stability, surface PEG density, drug encapsulation capacity and biomolecule immobilization capacity.
Owner:SHOICHET MOLLY S +2
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