42 results about "L-ascorbic acid 2-phosphate" patented technology

The invention provides a composition and a method for inducing mesenchymal stem cells to be differentiated to cartilage cells. The composition comprises 20-80 uM of L-ascorbic acid-2-phosphate, 2-10 ng/ml of TGF beta 3, 50-150 nM of dexamethasone, 10-50 ug/ml of vitamin C, 10-50 ug/ml of proline, 0.5-5 ug/ml of indometacin, 1xITS+1premix and 0.5-10 ug/ml of chlormadinone ester. The combination when being used as an inducer of a culture medium can improve differentiation activity and differentiation directionality.

The invention discloses an Australia murray cod puffing compound feed and a preparation method thereof. The Australia murray cod puffing compound feed comprises the following components in parts by weight: 60-70 parts of imported fish meal, 15-18 parts of special first-grade flour, 1-3 parts of bean pulp, 1-4 parts of beer yeast, 2-4 parts of inkfish liver meal, 0.05-0.2 part of methionine, 0.1-0.3 part of lysine, 0.2-0.4 part of glycine betaine, 1-3 parts of composite multiple vitamins, 1-3 parts of composite multiple mineral substances, 0.2-0.4 part of L-ascorbic acid-2-phosphate, 1-3 parts of monocalcium phosphate, 0.2-1 part of silicon type choline chloride, 2-6 parts of soya bean lecithin, 3-7 parts of imported fish oil, 0.1-0.5 part of yeast cell wall and 0.1-0.4 part of L-carnitine. After the Australia murray cod puffing compound feed is puffed at a high temperature, the digestibility is greatly improved, a large quantity of bacteria are killed at a high temperature, the transmission of diseases is avoided, the puffed feed is compact and low in loss percentage of dry matters, the pollution of feed loss to a water body is avoided, and the Australia murray cod puffing compound feed is rich in nutritional ingredients.

The invention discloses a method for measuring alkaline phosphatase activity. According to the method, L-ascorbic acid-2-phosphate is taken as a catalytic substrate of alkaline phosphatase, the substrate is hydrolyzed to form L-ascorbic acid under the catalysis of alkaline phosphatase, L-ascorbic acid can reduce bivalent copper into monovalent copper, a bright yellow bivalent copper-bathophenanthroline compound is converted into deep green monovalent copper-bathophenanthroline compound, the higher the alkaline phosphatase activity is, the more obviously the color of a detection liquid changes, and accordingly, semiquantitative colorimetric detection of the alkaline phosphatase activity is realized or quantitative detection is realized through an ultraviolet-visible spectrophotometer. By means of the method, alkaline phosphatase with limit of detection being 3.05 mU*mL<-1> can be detected. Compared with conventional methods, the method has high sensitivity and anti-interference capability and good repeatability, and is simple to operate, short in detection time and low in cost, and a simple, convenient, low-cost, high-resolution and sensitive colorimetric analysis method with stable performance is provided for detection of the alkaline phosphatase activity in a clinic sample.

The invention discloses an inducer and a method for inducing differentiation of adipose-derived stem cells to cartilage cells, belonging to the technical field of cell biology. According to the technical scheme, the inducer comprises TGF-Beta 3, FGF-18, dexamethasone, insulin, selenium, transferrin, linoleic acid, BSA, sodium pyruvate solution, L-ascorbic acid-2-phosphate and proline. The inducer and the method have the advantages that the synthesis and metabolism of the cartilage cells are rapidly promoted, the generation of cartilage is promoted, the expression of cartilage extracellular matrix II type collagen and glycosaminoglycan is remarkably improved, and the time required for in-vitro induction culture is reduced; and the inducer and the method are widely suitable for various culture systems, especially a pellet system and a three-dimensional cell scaffold culture system.

The invention discloses a method for preparing L-ascorbic acid-2-phosphate. The preparation method comprises the following steps: taking VC raw powder, sodium trimetaphosphate, calcium chloride and calcium hydroxide as raw materials to perform catalytic esterification reaction; and standing and curing a catalytic esterification product for 18+/2 hours, and drying the product to obtain the L-ascorbic acid-2-phosphate. The L-ascorbic acid-2-phosphate has the characteristics of large specific gravity of materials, small water solubility and the like, and is particularly suitable for compound feed of aquatic animals.

The invention discloses a quadruple linkage bad breath eliminating composition toothpaste which comprises an active ingredient consisting of the following components in percentage by weight: 0.5-1.5% of tea polyphenol, 0.5-1% of L-Ascorbic-acid-2-phosphate sodium, 1-3% of beta-cyclodextrin and 0.3-2% of a PVM/MA copolymer, and the balance being a toothpaste substrate. The toothpaste disclosed by the invention, through a linkage effect of four active ingredients, not only takes efficacy of single ingredient but also realizes a synergistic effect of all functional ingredients; by using the quadruple therapy to oral cavity, the toothpaste can inhibit odor inducing bacteria in the oral cavity through effective release of antibacterial ingredients in a process of brushing teeth; through an absorbent, bad breath in the oral cavity is absorbed; through absorption and film forming functions of the high-molecular copolymer, effective release of the active ingredients is guaranteed and residence time in the oral cavity is prolonged; meanwhile, a normal microorganism environment in the oral cavity is maintained, and effects of treating both symptoms and root causes and freshening breath for a long time are achieved, so as to meet requirements of people for improving images and pursuing healthy and green life.

A cosmetic containing L-ascorbic acid 2-phosphate sodium salt that excels in long-term stability. In particular, there is provided a cosmetic characterized in that L-ascorbic acid 2-phosphate sodium salt (A) and at least one compound (B) selected from the group consisting of arginine, urea and triethanolamine are contained in a mixing mass ratio of (A):(B) ranging from 1:0.001 to 1:0.5.

The invention relates to mixed feed suitable for grass carp in Ningxia regions and a preparation method of the mixed feed. The feed is mixed feed for grass carp which is prepared from the following raw materials of flour, bran, rice bran, bean pulp, cottonseed meal, degossypolized cottonseed protein, DDGS, rapeseed meal, fish meal, corn gluten meal, soybean oil, monocalcium phosphate, bentonite, zeolite powder, choline chloride, sodium chloride, compound premix feed for grass carp, L-ascorbic acid-2-phosphate and a micro-ecological preparation. The mixed feed has an excellent facilitating effect of improving the breeding level of grass carp in northwest regions. Meanwhile, high-quality feed raw materials and intestinal regulation micro-ecological preparations are further selected, so that the production process is continuously improved, and the feed has obvious effects of improving the food consumption of the grass carp, increasing the daily gain of the grass carp and improving the feed conversion ratio.

The invention discloses a feed additive for promoting the acute stress recovery of freshwater fish and application of the feed additive. The feed additive is characterized by comprising linseed oil, vitamin E and vitamin C which serve as effective components and consisting of the following raw materials in parts by weight: 35-45 parts of linseed oil, 0.04-0.06 part of vitamin E acetate, 0.10-0.20 part of L-ascorbic-acid-2-phosphate, 15-25 parts of glucose, 10-20 parts of glycine betaine, 5-15 parts of sodium chloride, 3-6 parts of algal polysaccharides, 3-5 parts of methionine, 3-5 parts of lysine and 0.01-0.02 part of ethoxy guin. The feed additive can be added into a feed according to the dosage of 5-10 percent. The feed additive has the advantages that under environmental stress, the survival rate of fish is improved by 20-30 percent after the fish is fed with the feed with the additive disclosed by the invention, the weight gain ratio is improved by 40-45 percent, and gill, liver and alimentary canal tissue structures, the blood condition, the anti-oxidization property, the immune response performance and the disease resistance can be recovered to a normal level.

One of the object of the present invention is to provide an L-ascorbic acid 2-phosphate metal salt, which causes no clouding and scarcely precipitates or deposits even when added to a cosmetic material or the like having blended therein an organic acid. Disclosed herein is an L-ascorbic acid-2-phosphate metal salt with a low calcium content characterized in that the content of a calcium compound is 500 ppm or less in terms of calcium ion, and production process thereof.

The invention discloses an active granular formula feed for feeding carp and a preparation method of the active granular formula feed. Every 100 parts of the active granular formula feed comprise the following components in part(s) by weight: 0.01-0.03 part of ferrous sulfate, 0.0012-0.01 part of anhydrous cupric sulfate, 0.0058-0.058 part of zinc sulfate, 0.0002-0.0004 part of vitamin A, 0.0001-0.0006 part of vitamin D, 0.1-0.3 part of L-ascorbic acid-2-phosphate, 0.2-0.4 part of hydroxy methionine, 0.02-0.1 part of bile acid, 0.1-0.3 part of montmorillonite, 0.01-0.04 part of bacillus subtilis, 0.05-0.1 part of an enzymic preparation, 0.03-0.05 part of taurine, 0.5-2.5 parts of monocalcium phosphate, 20-40 parts of bean pulp, 3-8 parts of alfalfa meal, 10-20 parts of imported fish meal, 1-2 parts of soyabean lecithin oil, 1-3 parts of other greases and the balance of wheat middling. As multiple vitamins and mineral substances are added to the active granular formula feed disclosed by the invention, the metabolism of the fishes is prompted, the survival rate at the early stage is increased, the growing speed at the late stage is increased, the fishes are kept healthy and grow rapidly, the feed coefficient is reduced, and great benefits are created for breeders.

A process for preparing L-ascorbate-2-phosphate and its salts, including L-ascorbate-2-monophosphate, L-ascorbate-2-polyphosphate and their salts, features the esterifying reaction between L-ascorbate and phosphuric acid esterifying agent in water. Said phosphoric acid esterifying agent is the mixture of P2O5 and the hydroxide or oxide of alkali-earth metal or hydroxide of alkali metal.

The invention discloses a compound feed capable of increasing red of the body color of aquarium fish and a preparation method thereof. According to weight parts, each 100 parts of compound feed comprise the following components in parts by weight: 0.01-0.3 part of ferrous sulfate, 0.0012-0.01 part of anhydrous cupric sulfate, 0.0058-0.058 part of zinc sulfate, 0.0002-0.0004 part of vitamin A, 0.0001-0.0006 part of vitamin D3, 0.1-0.3 part of L-ascorbic acid-2-phosphate, 0.5-2 parts of monocalcium phosphate, 15-25 parts of soya bean meal, 3-8 parts of corn protein meal, 3-10 parts of fish meal, 0.1-0.5 part of astaxanthin, 1-3 parts of seaweed meal, 3-8 parts of wheat germ powder, 1-3 parts of grease and the balance being flour. The compound feed selects the raw materials including the fish meal, the soya bean meal and the like to meet the nutrition needed by a fish body, so that the fish body is healthy; special substances including the corn protein meal, the seaweed meal, the wheat germ powder, the astaxanthin and the like are added to enable the fish body to be beautiful and the color to be bright and thus the ornamental value of the fish is improved.

The invention belongs to the technical field of cell culture of biological technique, and particularly relates to a serum-free medium for purifying myocardial cells of stem cell sources. The serum-free medium comprises the following components of 0.1-5mmol/L of L-glutamine, 2-10mmol/L of insulin, 0-800mug/mL of vitamin A, 100-300mug/mL of vitamin C (L-ascorbic acid-2-phosphate), 0.1-2ml/mL of gene reconstruction human serum albumin, 0.1-5% of amino acid, 1-5% of unnecessary amino acid, 0.1-0.5% of trace elements, lactose, quercetin, bromo deoxyribonucleoside, and RPMI (Roswell park memorial institute) sugar-free medium. The serum-free medium has the advantages that the myocardial cells of a mixing system can be purified, the ratio of other non-myocardial cells in a cell mixture is gradually decreased, and finally the myocardial cells with high purity can be obtained; the serum-free medium does not contain blood, so that the injury caused by virus pollution is avoided.

The invention discloses an L-ascorbic acid-2-phosphate compound and a preparation method and application thereof. The preparation method comprises the following steps: a, a second mixing procedure, namely mixing alkali liquor with first mixed liquor to prepare second mixed liquor with pH being 6.4-6.7; b, a third mixing procedure, namely mixing sodium trimetaphosphate with the second mixed liquor to obtain third mixed liquor; c, a fourth mixing procedure, namely mixing alkali liquor with the third mixed liquor to obtain fourth mixed liquor with pH being 11.0-11.5; and d, solidifying the fourth mixed liquor by standing and drying the solidified mixed liquor in microwaves with the frequency being 2400-2500MHZ to obtain the L-ascorbic acid-2-phosphate compound, wherein the first mixed liquor contains vitamin C, anhydrous calcium chloride, a stabilizer and water, and the alkali liquor is one or more of an aqueous solution of calcium hydroxide, an aqueous solution of sodium hydroxide and an aqueous solution of potassium hydroxide. The L-ascorbic acid-2-phosphate compound is excellent in stability.

The invention discloses a biological environmental-friendly poultry premix. The biological environmental-friendly poultry premix comprises the following raw materials of a microecological preparation,xylanase, a carrier, phytase, composite vitamins, methionine, an antioxidant, choline chloride, L-ascorbic acid-2-phosphate ester, organic copper, organic iron, organic zinc, organic manganese, iodate, organic selenium, iodized salt, calcium hydrogen phosphate and sodium bicarbonate. According to the premix, the cooperation effects of the phytase, the xylanase and the microecological preparationare utilized, and absorption and utilization efficiency of plant source feeds by birds can be improved, so that the dosage of a nutritive additive in a feed is reduced; and besides, all components inthe premix have synergistic effects, so that absorption and utilization efficiency of an additive in the premix by the birds can be improved, under the premise that the premix has better effects of promoting growth and development and egg production properties of the birds, resources are saved, the cost is reduced, environment pollution is reduced, the quality of bird products is improved, and thehealth of consumers is guaranteed.

The invention discloses a preparation method of a photoelectrochemical furazolidone sensor, and belongs to the technical fields of novel nano functional materials and biosensors. According to the preparation method, a novel dual metal co-doped two-dimensional photosensitizer is prepared at first, namely, iron and manganese co-doped titanium dioxide nano blocks and molybdenum disulfide carry out in-situ synthesis to generate a two-dimensional nano composite material (FeMn-TiO2/MoS2), which has a good biocompatibility and large specific area, then a furazolidone antibody is loaded on the two-dimensional nano composite material, alkaline phosphatase is fixed on the two-dimensional nano composite material; when the photosensitizer is used for detection, alkaline phosphatase can catalyze L-ascorbic acid-2-phosphate trisodium salt (AAP) to generate L-ascorbic acid (AA) in-situ; thus electron donors are provided for photoelectric detection; due to the influence of specific and quantitative combination between antibody and antigen on the electron transmission performance, the photoelectric current strength is correspondingly reduced, and finally a photoelectric sensor for detecting furazolidone through a label-free photoelectric method is manufactured.

The invention discloses a high-sensitivity and high-selectivity fluorescence analysis method for detecting specific DNA fragments by introducing morpholino oligonucleotides. Morpholine oligonucleotides covalently linked to the surface of magnetic microspheres are used as capture probes, and after hybridization with longer target DNA fragments, the unhybridized segments of target DNA are then hybridized with signal DNA with end-modified biotin, using strands The combination of mycoavidin and biotin introduces alkaline phosphatase into the signal probe DNA end, alkaline phosphatase catalyzes L-ascorbic acid 2-phosphate to generate L-ascorbic acid, and then reduces resazurin to form L-ascorbic acid Strong fluorescent resorufin, the fluorescence intensity is correlated with the target DNA, through the method of fluorescence analysis to achieve high sensitivity and high selectivity detection of DNA fragments, it can be used in molecular recognition, clinical diagnosis, environmental monitoring and food safety and other fields detection of DNA samples.

The invention belongs to the field of health products and in particular relates to a pharmaceutical composition for increasing bone density and a preparation method thereof. The pharmaceutical composition provided by the invention is prepared from the following raw materials in parts by weight: 2-6 parts of oysters, 0.5-2 parts of malt, 1-4 parts of Chinese yams, 1-3 parts of poria cocos, 1-4 parts of Chinese dates, 0.5-2 parts of liquorice, 0.5-2 parts of endothelium corneum gigeriae, 0.000001-0.000005 part of vitamin D2, 5-15 parts of calcium gluconate, 2-5 parts of sodium L-ascorbic-acid-2-phosphate, 1-4 parts of glutathione, 1-4 parts of phellinus igniarius fermented powder and 2-75 parts of auxiliary materials. The pharmaceutical composition provided by the invention has a good calcium supplementing effect, is rich in nutritional ingredients, safe and effective, convenient to take and applicable to being taken by children for a long time and can promote sustainable growth of the bone density.