The invention provides a non-flammable mixed refrigerant applicable to a temperature zone from -60 to -100 DEG C. The non-flammable mixed refrigerant comprises at least one selected from a group consisting of cis-1,1,1,4,4,4-hexafluorobutene, trans-1,1,1,4,4,4-hexafluorobutene, 1-chloro-3,3,3-trifluoropropene, cis-1,3,3,3-tetrafluoropropene, 1,1,1,2,2,3,4,5,5,5-decafluoropentane, perfluorobutane and perfluoropentane, at least one selected from a group consisting of perfluoropropane, pentafluoroethane, 1,1,2,2-tetrafluoroethane, 1,1,1,2-tetrafluoroethane, difluoromethane, 1,1,1-trifluoroethane, trifluoroiodomethane, 1,1,1,2,3,3,3-heptafluoropropane, 1,1,1,2,3,3-hexafluoropropane and 1,1,1,3,3-pentafluoropropane, at least one selected from a group consisting of trifluoromethane and perfluoroethane, and perfluoromethane. The non-flammable mixed refrigerant provided by the invention can safely and efficiently realize cooling in a temperature range of -60 to -100 DEG C, does not have flammability at all, and is low in overall greenhouse effect and free of ozone depletion effect.