The invention discloses beef soup, a preparation method thereof and a method for making a Chinese hamburger from the beef soup, and relates to the technical field of foods. The beef soup is prepared from ox bones, beef, beef offal, beef tallow, water, salt and a spice package, wherein the spice package comprises fennel, star anises, fructus tsaoko, radix angelicae dahuricae, nutmeg, fructus amomirotundus, semen alpiniae katsumadai, cassia bark, rhizoma kaempferiae, dried ginger, clove, radix et rhizoma nardostachyos and zingiberaceae seeds. The preparation method of the beef soup comprises the steps of putting the ox bones, the beef and the beef offal into a pan, adding water, performing boiling over with high heat, performing skimming, adding the spice package, performing boiling with low heat, and then adding the beef tallow and the salt, so as to obtain the beef soup. On the basis of traditional soup taste, the nutmeg, the rhizoma kaempferiae, the beef tallow, etc. are added, so that the boiled beef soup is small in peculiar smell, long in fragrance, and has good health-care and health-preserving functions, and the Chinese hamburger made from the beef soup and unique in flavorand balanced in nutrition can be taken with oneself and Leshan Qiaojiao beef is easy to taste.