The invention discloses bio-organic fertilizer. Through fermentation, the bio-organic fertilizer is prepared from 6-26wt% of filter mud produced by a sugar refinery, 55-75wt% of bagasse ash and 10-30wt% of ethanol concentrate. The production method comprises the following steps of 1, carrying out mixing stacking on filter mud produced by a sugar refinery, bagasse ash and ethanol concentrate, 2, removing moisture by filtration, 3, stacking the raw material mixture in a strip-shaped stack way, adding bifidobacterium, lactic acid bacteria, bacillus, saccharomycetes, actinomycetes and acetic bacteria into the stacked mixture, and carrying out fermentation, 4, keeping the primary fermented stack temperature above 60 DEG C and keeping material moisture content in a range of 40-45%, 5, increasing the height of the primary fermented stack, and carrying out secondary fermentation for aging until material moisture content is in a range of 30-35%, 6, adding the aged material into organic raw materials of N, P and K and carrying out mixing and 7, packaging the mixture to obtain the product. The bio-organic fertilizer has good fertilizer efficiency and is conducive to absorption by crops. The production method utilizes simple production equipment, has simple production processes and can be operated easily. The production method reduces a cost and has high income.