The invention discloses a quality control material for metabolomics detection and a quality control method of the quality control material. The quality control material comprises mixed standard samples of 4,4'-methylene bis(2-chloroaniline), p-anisidine, L-tyrosine methyl ester, 3-chloroaniline, 2,4-dimethyl quinoline, sulfapyridine, atrazine, sulfadoxine, DL-leucine, N-benzoyl-L-tyrosine ethyl ester, 6-phenyl-2-thiouracil, N-(o-toluoyl) glycine, 2-methyl-5-nitroimidazole-1-ethanol, glycyrrhetinic acid, flavanone, Epsilon-caprolactone and 2-aminopyridine. 17 standard samples in the method comefrom different material categories and are very stable, simple in preparation and small in amount; and the quality control material disclosed by the invention can accurately reflect the state of a chromatograph or a mass spectrometer.