2 results about "Peptide formation" patented technology

This invention discloses a system for rapid detection of tumor cell invasiveness and risk level reference based on matrix remodeling fluorescence reporter assay. After staining, test cells are seeded on a matrix material formed by cell adhesion ligands and fluorescent dye-modified polyisocyanate peptides for 2D culture, or the two components are thoroughly mixed and gelled to form a 3D culture environment, resulting in a cell-induced matrix remodeling fluorescence detection system. Fluorescence images of the area surrounding the sample are acquired, and cell invasiveness risk is analyzed based on changes in the intensity of the fluorescence signal in the surrounding area and the matrix remodeling index. This invention provides a more direct and rapid assessment of tumor cell invasiveness by observing the matrix remodeling characteristics induced during cell invasion in real time in vitro, providing a risk level reference for tumor malignancy.

This invention discloses a membrane-modified liposomal drug targeting CD177-positive neutrophils and its preparation method. The membrane-modified liposomal drug has a three-layer structure, including: a liposome loaded with a PADI4 inhibitor, a targeting recognition layer formed by coupling a CD177 targeting peptide to the surface of the liposome, and an outermost biomimetic functional layer formed by coating the natural neutrophil membrane. Based on the key pathological mechanism of the "CD177-NETs axis," this invention designs a structurally sound, safe, and stable biomimetic targeted nanoformulation. It creatively combines the anti-inflammatory / chemotactic function of the neutrophil membrane, the precise targeting of the CD177 peptide to pathogenic subgroups, and the source blocking of NETs by the PADI4 inhibitor, achieving highly efficient dual-targeted enrichment and synergistic therapy.