97 results about "Phenylalanine methyl ester" patented technology

The invention relates to a fruit paste-containing yoghurt, which is prepared from 0.5-2.0 kg of xylitol, 5-20 kg of fruit grain or jam, 0.1-10 kg of thickener, 0.01-0.05 kg of aspartyl-phenylalanine methyl ester (containing phenylalanine), 0.01-0.05 kg of Acesulfame K (Acesulfame Potassium), 0.01-0.03 kg of edible spice, and fresh milk and leaven in balancing amount per 100 weight parts of the yoghurt. The invention also provides a method for preparing the fruit paste-containing yoghurt. The fruit paste-containing yoghurt has low sucrose content, and abundant nutrition and good taste by adopting various fruits. BY adopting the preparation method, the fruit paste-containing yoghurt with different flavors can be prepared, and are suitable for long-term consumption of consumers.

The present invention provides a synthetic method of Neotame, and relates to a sweetener, in particular to a method used for synthesizing the sweetener, Neotame, through the reaction of aspartame, 3, 3-dimethyl butyraldehyde and formic acid. And the present invention provides a synthetic method of Neotame, which has the advantages of simple process and cheap raw materials. The aspartame and the formic acid are added into methanol to prepare mixture A; the weight ratio of the methanol and the aspartame is equal to from 3 to 9 :1; the molar ratio of the formic acid and the aspartame is equal to from 1.2 to 3.0 :1; the 3, 3-dimethyl butyraldehyde is added in the mixture A; the molar ratio of the 3, 3-dimethyl butyraldehyde and the aspartame is equal to from 1.1 to 2.0 :1; after the reaction, the product is concentrated under the condition of reduced pressure to form pulp; and ethanol solution is used for recrystallization to prepare the Neotame.

A salt substitute composition is provided. The salt substitute composition has an amount of potassium chloride and an amount of N-[N-(3,3-dimethylbutyl)-L-α-aspartyl]-L-phenylalanine 1-methyl ester or neotame. The N-[N-(3,3-dimethylbutyl)-L-α-aspartyl]-L-phenylalanine 1-methyl ester is present in an amount of about 0.001 wt % to about 0.02 wt % of the total weight of the composition. The amount of N-[N-(3,3-dimethylbutyl)-L-α-aspartyl]-L-phenylalanine 1-methyl ester is optimized for reducing the undesired aftertaste of the potassium chloride.

The invention discloses an environment-friendly mixed feed for pigs and a preparation method thereof. The environment-friendly mixed feed for pigs is prepared from the following ingredients in part by weight: the environment-friendly mixed feed is prepared from corn, bean pulp, wheat bran, DDGS (distillers dried grains with soluble), acyl hydroxylation emulsified oil powder, calcium hydrogen phosphate, mountain flour, enzyme preparation, calcium propionate, citric acid, lysine, methionine, threonine and pre-mixture; and functional additives in the premixture comprise histidine, arginine, leucine, valine, corynebacterium glutamicum, L-asparaginate-L-phenylalanine methyl ester, spiral seaweed, glucanase, bacillus subtilis and sodium butyrate. According to the feed, engineering bacteria is utilized to generate glutamic acid through fermentation in an intestinal tract of the pig, the additional amino acids in the feed are balanced, all amino acids are induced to be combined in the small intestine in an ideal amino acid mode to be absorbed, the protein level of the feed and the carbon-nitrogen emission are reduced, a part of amino acids are promoted to be deposited in pork, the pork flavor is improved and the pork quality is improved.

In the separation and purification of N-[N-(3,3-dimethylbutyl)-L-alpha-aspartyl]-L-phenylalanine methyl ester produced in the reductive alkylation of aspartame (APM) with 3,3-dimethylbutylaldehyde, the APM used in the starting material for the alkylation, which is difficult to be separated by recrystallization only from the crude crystals of the reaction product, can be separated and removed by extraction or solution thereof with the specified organic solvent from the concentrated reaction solution of the reductive alkylation or from the crude crystals of the product therefrom, and further N,N-di(3,3-dimethylbutyl)-APM as the by-product can be separated and removed by means for separation and purification, such as crystallization, chromatography, extraction, treatment with the activated charcoal, etc. As a result, N-[N-(3,3-dimethylbutyl)-L-alpha-aspartyl]-L-phenylalanine methyl ester which is a sweetener having a high degree of sweetness can be purified efficiently, and also the APM derivative in a high purity is possibly produced industrially.

The invention belongs to the technical field of environmental functional material preparation, and relates to a method for preparing GQDs (graphene quantum dots) by a SBA-15 micro-reactor and an application of a fluorescent sensor. With SBA-15 taken as a micro-reactor, the problems of low yield, large size difference and the like in preparation of the GQDs in the prior art are solved, and the GQDs with uniform size and high quantum yield are prepared; the fluorescence sensor (BPA-MIP) capable of specifically recognizing BPA is prepared from MMA (methyl methacrylate) and MAP (methyl 3-phenyl-L-alaninate) as functional monomers and BPA as a template by free radical polymerization. Due to combination with a surface molecular imprinting technology, the recognition capacity of the GQDs for target molecules is improved, the response speed to target objects is increased, and a solid theoretical and practical foundation is laid for rapid and selective detection of trace BPA in beverages.

The invention belongs to the field of food additives, particularly relates to a sweetening agent, and more particularly relates to a method for synthesizing neotame through catalytic hydrogenation of N-alpha-L-aspartic phthalein-L-phenylalanine 1-methyl ester and 3,3-dimethyl butyraldehyde. A synthetic method of neotame is characterized in that Pd / C is taken as a catalyst to perform the catalytic hydrogenation of an ethanol solution of N-alpha-L-aspartic phthalein-L-phenylalanine 1-methyl ester and 3,3-dimethyl butyraldehyde so as to synthesize neotame. The synthetic method disclosed by the invention has the beneficial effects that neotame prepared by the method disclosed by the invention has a yield as high as 83% and liquid-phase purity as high as 98.7%; moreover, in comparison with the synthetic method in the background art, the method disclosed by the invention is beneficial for industrial production, low in cost and simple in experimental procedures.

A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.

It has now been found that N-L-alpha-aspartyl-L-phenylalanine 1-methyl ester (APM) and / or one of its lower alkyl derivatives can be used to treat allergic contact dermatitis associated with irritating oils such as catechol-containing plant-derived antigens such as poison ivy, poison oak, poison sumac and Asian lacquer tree and oils containing capsaicin. Topical application of APM and / or derivative can reduce or alleviate the symptoms associated with irritation of the skin and / or mucous membranes caused by contact or inhalation of these oils or fumes from burning vegetation containing these oils.

The invention provides a chiral N-heterocyclic carbene precursor salt with a 3,4-dihydroisoquinoline skeleton, a synthetic method and application. The precursor salt has the structural formula as shown in the specification. The precursor salt can be prepared by using cheap and readily available chiral phenylalanine methyl ester hydrochloride as a starting material, has good application in the asymmetric reaction under the catalysis of N-heterocyclic carbene, and can be used for synthesizing naphthopyrone compounds with high enantioselectivity.

The present invention provides a synthetic method of Neotame, and relates to a sweetener, in particular to a method used for synthesizing the sweetener, Neotame, through the reaction of aspartame, 3, 3-dimethyl butyraldehyde and formic acid. And the present invention provides a synthetic method of Neotame, which has the advantages of simple process and cheap raw materials. The aspartame and the formic acid are added into methanol to prepare mixture A; the weight ratio of the methanol and the aspartame is equal to from 3 to 9 :1; the molar ratio of the formic acid and the aspartame is equal tofrom 1.2 to 3.0 :1; the 3, 3-dimethyl butyraldehyde is added in the mixture A; the molar ratio of the 3, 3-dimethyl butyraldehyde and the aspartame is equal to from 1.1 to 2.0 :1; after the reaction,the product is concentrated under the condition of reduced pressure to form pulp; and ethanol solution is used for recrystallization to prepare the Neotame.

The invention provides an injected amino acid derivative micromolecule gel material and application thereof, and belongs to the field of biomedical engineering in material chemistry. A preparation method comprises the following steps that first, phenylalanie methyl ester (D / L type) is regarded as a reaction zymolyte, and is modified by 1,4-naphthalic acid (NDC) and diethylene glycol, and amino acid derivative micromolecule gelator NDC-L or / and D-PhOMe, NDC-L or / and D-PhOH; and then, the gelator is added into polyethylene glycol, and heated to be completely dissolved, then equivoluminal deionized water is added, and after mixing, the injected amino acid derivative micromolecule gel material is obtained for several-minute standing. According to the injected amino acid derivative micromolecule gel material and the application thereof, a procedure is relatively simple, operation is convenient, the prepared gel material after freezing and drying is of a three-dimension (or nostoc commune) structure, biocompatibility is good, degradation is easy, the slow-release effect of medicine vitamin B12 is excellent, the gel material can be regarded as a medicine support or vector used for clinic treatment, and great practical prospects are achieved.

The invention provides a preparation method of amino protected L-3-formyl-4-methoxyl-5-methyl-phenylalanine methyl ester (structural formula is below), highly effectively converts the amino protected L-3-formyl-4-methoxyl-5-methyl-phenylalanine methyl ester into amino protected L-3-hydroxyl-4-methoxyl-5-methyl-phenylalaninol / phenylalanine, and develops a simple and practical new method for synthesizing a L-3-hydroxyl-4-methoxyl-5-methyl-phenylalaninol / phenylalanine compound. The L-3-hydroxyl-4-methoxyl-5-methyl-phenylalaninol / phenylalanine compound is an important intermediate for chirally synthesizing tetrahydroisoquinoline alkaloids with high anti-cancer activity such as Ecteinascidin 743, Saframycin A and Cribrostatin IV. The method overcomes the disadvantages of the prior synthesis, has the advantages of short steps, high yield, cheap raw material, moderate conditions, simple and convenient operation, and easy industrial production, and is a good method for preparing the L-3-hydroxyl-4-methoxyl-5-methyl-phenylalaninol / phenylalanine compound.

The invention provides a modification method on the basis of crosslinking reaction between laccase amino acid residue terminal carboxyl group (-COOH) and amino group of methyl L-phenylalaninate hydrochloride to enhance activity and stability of the laccase, which comprises the following step: mixing laccase, methyl L-phenylalaninate hydrochloride and 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride at a certain temperature to react to obtain the modified laccase. Compared with the unmodified laccase, the modified laccase has higher activity and stability. The modified laccase can be used for aftergrowth fiber modification, and can save the laccase consumption on the premise of enhancing the strength property of the paper sheets made from aftergrowth fibers.

The invention relates to an organic porous gel absorbent for quick treatment of dye wastewater in printing and dyeing industry, a preparation method and application of the organic porous gel absorbent. The organic porous gel absorbent is 1,4-phenyl phenylalanine. The preparation method comprises the steps of preparing 1,4-phenyl phenylalanine methyl ester from phthaloyl chloride, L-phenylalanine methyl ester hydrochloride and triethylamine; and preparing the dyeing absorbent 1,4-phenyl phenylalanine from 1,4-phenyl phenylalanine methyl ester. The invention also relates to the application of the organic porous gel absorbent as a quick absorbent in dye wastewater treatment. The organic porous gel absorbent provided by the invention is a low-molecular-weight gel absorbent with an active carbon porous structure, and can rapidly and efficiently capture dyes in the industrial wastewater through phi-phi interaction, with the advantages of easy recovery and low cost. The dye adsorption can beconducted by simple operation at normal temperature, so that the organic porous gel absorbent has actual application value.

The present invention relates to a process for the preparation of nateglinide, preferably in B-form, substantially free from the H-form, comprising three steps starting from D-phenylalanine methyl ester or a salt thereof.

The invention discloses a novel phenylisoserine derivative: (2R, 3S)-3-(tert-butoxycarbonylamino)-2(2-ethoxypropene-2-oxo)-3-phenylpropionic acid as a side chain for the synthesis of docetaxel, and a preparation method for the compound. The preparation method includes the following steps that: (2R, 3S)2-hydroxy-3-amino phenylalanine methylester is prepared with (3R, 4S)3-hydroxy-4-phenylazetidine-2-ketone ring-opening product, the amino group is protected by the tert-butoxycarbonyl group, the hydroxyl group is protected by the 2-ethoxypropene group, the methyl ester is unshackled, and thereby the novel phenylisoserine derivative is obtained. The invention also discloses a midbody compound which is prepared with the compound and used for synthesizing docetaxel, and a preparation method for the midbody compound. The novel synthesis route for synthesizing docetaxel has the advantages of mild reaction conditions and high condensation efficiency, can be used for industrialized production, and can be widely popularized.

Of the sugar-less buckwheat flavone beverage, each 1000 ml contains buckwheat flavone or rutin in 50-80 mg, xylitol 5-15 g, sodium cyclohexyl amino sulfonate in 0.2-0.6 g, potassium carbonate 5-10 mg, citric acid in 0.05-0.2 g, and pure water for the rest. The sodium cyclohexyl amino sulfonate may be also replaced with N-L-alpha-aspartyl-L-methyl phenprobamate in 0.05-0.15 g. As one kind of natural functional beverage, the present invention is especially suitable for middle-aged people and old people.

The present invention is one-step enzyme process of synthesizing aspartame, and the one-step enzyme process is developed on the basis of available biological synthesizing process. The one-step enzyme process of synthesizing aspartame includes fermentation with some strain to obtain enzyme and the subsequent direct synthesis of aspartame with aspartic acid, phenylalanine and methanol as substrate. The present invention has reduced reaction steps, simplified production process and lowered production cost.

The invention provides an apple-flavor lactobacillus beverage, and relates to the field of processing of food beverages, in particular to a formula of a milk beverage and a preparation method of the formula of the milk beverage. The apple-flavor lactobacillus beverage consists of whole milk powder, water, apple juice, sugar, a food additive, probiotics and a thickening agent; the apple juice is concentrated apple juice; the sugar is white granulated sugar, high fructose corn syrup or one or a combination of sweetener neotame, sodium cyclamate, sucralose, phenylalanine methyl ester accesulfame, stevioside and acesulfame potassium. The milk, the apple and the probiotics are combined to form the lactobacillus beverage which is novel and has flavor of apple. Compared with a like lactobacillus beverage, the obtained lactobacillus beverage has obvious market advantages; large profit margin is created for enterprises; and moreover, the produced lactobacillus beverage is suitable for people of all ages, and is quite wide in market prospect.

The improved A-NK cell separating culture method is one improved tumor treating biologically therapeutic method, and includes adopting PME to eliminate mononuclear phagocyte without altering phynotype and toxicity of lymphocyte; displacing no-serum culture medium for serum culture medium to obtain similar number and function of the cultured matter and raised clinical safety; combining IL-4 with IL-2 to generate relatively strong stimulation to A-NK cell growth and mature and to raise the killing activity of the activated immunological cell.

An improved method is described for the synthesis of aspartame using a condensation reaction between the alpha-carboxyl group of the L-aspartic acid and the amino group of the methyl L-phenylalaninate catalyzed by an enzyme. The method allowed efficient and cost effective production of aspartame. A method of identifying an enzyme useful for preparing aspartame is also described.

The invention discloses a preparation method of N-(pyrazine-2-radical carbonyl)-L-phenyl alanine. The preparation method comprises the following steps of: (1) making thionyl chloride react with pyrazine-2-formic acid in an organic solvent to obtain pyrazine-2-formyl chloride; (2) making thionyl chloride react with L-phenyl alanine in methanol to obtain L-phenyl alanine methyl ester hydrochloride; (3) dissolving pyrazine-2-formyl chloride and a metal M in tetrahydrofuran and adding the solution into a tetrahydrofuran solution of the L-phenyl alanine methyl ester hydrochloride and the metal M to obtain N-(pyrazine-2-radical carbonyl)-L-phenyl alanine methyl ester; and (4) dissolving the N-(pyrazine-2-radical carbonyl)-L-phenyl alanine methyl ester in an organic solvent and hydrolyzing under an alkaline condition to obtain N-(pyrazine-2-radical carbonyl)-L-phenyl alanine. A synthetic method has the advantages of less operation steps, high yield, readily-available raw materials and low environmental pollution, and is suitable for industrial production.

The invention relates to the technical field of chemical engineering, in particular to a preparation method of aspartame-acesulfame salt. The preparation method of the aspartame-acesulfame salt comprises the following steps of: mixing an aspartame aqueous solution with acesulfame, regulating pH (Potential of Hydrogen) to 1.2 to 4.0, and mixing for reacting under the condition that the temperatureis 20 to 65 DEG C, thus forming the aspartame-acesulfame salt. By adopting the aspartame aqueous solution and controlling the pH value and the reaction temperature, remaining of potassium chloride canbe effectively avoided, and the yield is ensured; the preparation method is simple and convenient, equipment requirements are simple, the yield of obtained products is 90 percent or above, the cost is low, and the preparation method is suitable for industrial production.

The invention discloses a supramolecular hydrogel factor and a preparation method thereof. The supramolecular hydrogel factor molecularly comprises a ferrocene unit and two phenylalanine units. The ferrocene dicarboxylate and phenylalanine methyl ester hydrochloride are condensed under the action of an amide condensing agent and hydrolyzed under alkaline conditions to obtain the supramolecular hydrogel factor. The invention further discloses supramolecular hydrogel. The supramolecular hydrogel is obtained by self-assembly of the supramolecular hydrogel factor in a buffer solution with pH being 3-5 through noncovalent interaction. The supramolecular hydrogel has multiple stimulative responsibilities including pH responsibility, temperature responsibility, electrochemical responsibility and the like and is high in biocompatibility. Preparation methods of the supramolecular hydrogel factor and the supramolecular hydrogel are short in technical process, simple in operation, mild in reaction condition and high in reaction yield.

The invention discloses a strawberry flavor sour milk beverage, relates to the field of food beverage processing, and particularly relates to a recipe containing a lactic acid beverage and a preparation method of the recipe. The strawberry flavor sour milk beverage is prepared from the following raw materials: fermented sour milk, water, strawberry juice, strawberry essence, sugar, a food additive, probiotics, a thickener, citric acid and a compound stabilizer, wherein the sugar is one or a combination of white granulated sugar, high fructose corn syrup or a sweetening agent neotame, sodium cyclamate (alternate name: Tianmisu), sucralose, aspartame-acesulfame salt, stevioside and acesulfame potassium (alternate name: Ansaimi). Afther the technical scheme is adopted, the strawberry flavor sour milk beverage has the beneficial effects that the sour milk beverage is prepared from the fermented sour milk, the strawberry juice and the probiotics, so the structure of a sour milk beverage product is broken through, and product types are enriched; the strawberry juice is added in the sour milk beverage, so that a product has both unique flavor of yoghourt and good mouth feel of strawberry, and the nutrition value is greatly improved.

There is provided a process for purification of N-(3,3-dimethylbutyl)-alpha-L-aspartyl-L-phenylalanine methyl ester comprising the step of contacting impure N-(3,3-dimethylbutyl)-alpha-L-aspartyl-L-phenylalanine methyl ester containing at least alpha-L-aspartyl-L-phenylalanine methyl ester as an impurity, with the mixed solvents having two layers of one layer given from at least one organic solvent which does not mix with water homogeneously and one layer of water to extract N-(3,3-dimethylbutyl)-alpha-L-aspartyl-L-phenylalanine methyl ester in the organic solvent layer.In the present invention, the desired compound can be purified or recovered efficiently in a high yield without using burdensome steps or operations.

The invention provides a bisoxazoline ligand compound and a synthetic method thereof. The method comprises the following steps: synthesizing (tert-butoxycarbonyl)-L-phenylalanine methyl ester 2 by using L-phenylalanine methyl ester hydrochloride 1, synthesizing (S)-(3-hydroxy-3-methyl-1-phenylbut-2-yl)tert-butyl carbamate 3 or (S)-(3-butyl-3-hydroxy-1-phenylhept-2-yl)tert-butyl carbamate 5, synthesizing (S)-3-amino-2-methyl-4-phenylbutan-2-ol 4 or (S)-3-amino-2-methyl-4-phenylbutan-2-ol 6 by using the above materials, and finally, synthesizing the compound provided by the invention. The preparation method provided by the invention is simple, and has easy purification and a higher yield; and compared with a PyboxLigand, the substance prepared by the method realizes the purpose that carbon connected to an oxygen atom is connected with a substituent, and the substance synthesized by the method has a better ee value and better applications in asymmetric synthesis.

The invention discloses an environment-friendly fountain solution, which is characterized by being prepared from the following ingredients in parts by weight: 2 to 10 parts of polyoxyethylene isooctyl ether phosphate, 2 to 8 parts of corn starch, 1 to 3 parts of ammonium bicarbonate, 0.5 to 1.5 parts of trihydroxymethyl aminomethane, 1 to 3 parts of nicotinamid, 1 to 3 parts of sodium octane sulfonate, 1 to 3 parts of castor oil, 2 to 8 parts of castor oil maleic acid ester, 0.5 to 1.5 parts of tea polyphenol, 1 to 5 parts of lactic acid, 1 to 4 parts of L-Aspartyl-L-phenylalanine methyl ester, 1 to 2 parts of tea tree essential oil, 5 to 10 parts of auxiliaries and 30 to 50 parts of water. The fountain solution disclosed by the invention has the advantages that the raw material resource is rich, the cost is low, the wetting effect is good, volatile and irritant substances are not contained, the cost is reduced, the fountain solution is mild and harmless to human bodies and environments, and the requirements of energy saving and environment protection are met.