3results about How to "Effective transformation" patented technology

The present application relates to the field of microbial technology, in particular to a recombinant microorganism and a construction method and application thereof. The present application provides a recombinant microorganism, wherein the recombinant microorganism has reduced expression and / or enzymatic activity of a mechanosensitive protein YnaI or a homolog or a functional variant thereof compared with a starting strain. The present application significantly improves the yield and conversion rate of threonine and other amino acids by reducing the expression of the mechanosensitive protein YnaI in the microorganism. The yield and conversion rate of threonine of the recombinant microorganism provided by the present application with reduced expression of the mechanosensitive protein YnaI are significantly improved compared with the starting strain, which is conducive to reducing the fermentation production cost of threonine and provides an effective modification target and strain for the breeding of a high-yield threonine strain.

This invention relates to the field of microbial technology, specifically to recombinant microorganisms for producing threonine, their construction methods, and methods for producing threonine. This invention discovers that reducing the expression of the β-phosphoglucomutase YcjU in microorganisms can significantly increase the yield and conversion rate of threonine and other amino acids. The recombinant microorganisms with reduced β-phosphoglucomutase YcjU expression provided by this invention exhibit significantly higher threonine yield and conversion rate compared to the starting strain, which is beneficial for reducing the fermentation production cost of threonine and provides effective modification targets and strains for the breeding of high-yielding threonine strains.

The application discloses a kind of low permeability fluorine pollution sediment repair methods, comprising: S1, citric acid, diethylene triamine pentaacetic acid and coconut amide propyl betaine three solutions are mixed in proportion, to obtain leaching agent;S2, fluorine pollution sediment is repaired: to fluorine pollution sediment, leaching agent obtained in S1 is added, continuously stirring 1~2h in leaching stirring tank;In the process of leaching, gas is pumped from the porous disc at the bottom of leaching stirring tank using pressurized air pump, and the fluorine pollution sediment saturated with leaching is repaired using micro-bubble-leaching combined enhancement repair formed by micro-bubble;S3, the sediment after S2 repair is separated, and the leaching is completed.The application has the advantages of simple process, convenient operation, fast reaction speed, good leaching effect, easy to obtain leaching agent and micro-bubble device, and has combined enhancement effect on fluorine pollution sediment repair, and is suitable for fluorine pollution repair of low permeability sediment such as clay, silty clay and silt.