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The present application relates to the field of microbial technology, in particular to a recombinant microorganism and a construction method and application thereof. The present application provides a recombinant microorganism, wherein the recombinant microorganism has reduced expression and / or enzymatic activity of a mechanosensitive protein YnaI or a homolog or a functional variant thereof compared with a starting strain. The present application significantly improves the yield and conversion rate of threonine and other amino acids by reducing the expression of the mechanosensitive protein YnaI in the microorganism. The yield and conversion rate of threonine of the recombinant microorganism provided by the present application with reduced expression of the mechanosensitive protein YnaI are significantly improved compared with the starting strain, which is conducive to reducing the fermentation production cost of threonine and provides an effective modification target and strain for the breeding of a high-yield threonine strain.