The invention provides a fabrication process for a high-grade pure cotton shirt. The fabrication process comprises the steps of tailoring, sticking a collar lining, ironing a front fly, ironing pockets, pulling complex potential, sewing up small shoulders, making a top collar, reversing the top collar, making a lower collar, making a three-clamp collar, assembling the collar, sewing sleeve vents, assembling sleeves, sewing side seams by hands, sewing sleeve bottom seams, assembling cuffs, coiling bottom sides, locking holes, sewing buttons, ironing, inspecting finished products and the like. The fabrication process provided by the invention detailedly and concretely standardizes each process sequence and concrete parameters in the fabrication course of the high-grade shirt, at the same time, in the fabrication process, handwork and machines are combined together, the fabrication and production efficiency of the shirt is ensured, and the quality of the shirt is further ensured at the same time, so that the shirt made by the technical scheme provided by the invention is excellent in the quality and patterns, the wearing effect is upgraded, and the shirt is upscale.