The invention relates to an ultrathin secondary battery and a preparation method thereof, and belongs to the field of electrochemistry. The thickness of the ultrathin secondary battery is 0.3-1.0mm. The ultrathin secondary battery comprises a diaphragm, a positive electrode membrane and a negative electrode membrane, wherein the positive electrode membrane and the negative electrode membrane are respectively adhered to two sides of the diaphragm and directly contact the diaphragm; the diaphragm consists of a substrate and a conductive layer; the conductive layer is positioned on one side of the substrate and directly contacts the positive electrode membrane. The preparation method comprises the following steps: firstly, respectively coating the front and back sides of the diaphragm with positive electrode slurry and negative electrode slurry according to set sizes, drying and rolling, thereby obtaining the diaphragm with the positive electrode membrane and the negative electrode membrane; secondly, pressing, thereby enabling a positive electrode current collector and a negative electrode current collector to respectively tightly contact the positive electrode membrane and the negative electrode membrane; finally, packaging by using a package membrane according to design size, performing vacuum dewatering and electrolyte injection in sequence, subsequently sealing, activating, forming, packaging for the second time, and performing volume division, thereby obtaining the ultrathin secondary battery.