The invention discloses a formula and a preparation method of a special synergist for soil improvement. The formula comprises the following raw materials in percentage by weight: 1-6% of phosphorite, 2-8% of magnesium silicate mineral, 4-9% of potassium sulfate, 5-10% of compound amino acid, 4-9% of urea, 2-6% of enzyme microorganisms, 1-8% of urea formaldehyde, 3-9% of metalloproteinase, 13-18% of humic acid, 14-20% of lime,11-20% of crop straw, 14-22% of chicken manure, 0.2-2% of biological agent, 0.1-1% of urease inhibitor, 0.2-1% of ammoniastabilizer, 0.4-0.8% of DCPTA, 0.1-0.2% of hydrogen peroxide silver ion solution, and 0.2-0.6% of sepiolite. According to the formula of the special synergist for soil improvement, the metalloproteinase is added, the metalloproteinase contains long-chain protease and an anionic surfactant, nitrogen release is controlled, water-soluble phosphorus fixation is inhibited, microelements are collected, the validity period of the synergist is prolonged, the synergistic effect is remarkably improved, and the polypeptide component in the metalloproteinase can play roles in rooting, root nourishing and root strengthening. The enzyme microorganisms are added and are beneficial to fermentation and decomposition of organic materials, and soil is helped to decay crop residues in the last season.