The invention discloses a nursing disinfectant for the obstetrics and gynecology department and a preparation method of the nursing disinfectant. The nursing disinfectant is prepared from a traditional Chinese medicine extract, saffron essential oil, menthol, sodium chloride, terbinafine hydrochloride and sterile water, wherein the traditional Chinese medicine extract is prepared from milkvetch roots, liquorice roots, plantain herbs, christina loosestrife herbs, butterflybush flower, wild chrysanthemum flowers, myrrh, dandelion, folium artemisiae argyi, danshen roots, lotus seeds, climbing groundsel herbs, suberect spatholobus stems, thorny elaeagnus leaves and divaricate knotweed herbs through two times of water extraction and concentration, the traditional Chinese medicine extract is dissolved in a proper amount of sterile water, the saffron essential oil, menthol, sodium chloride and terbinafine hydrochloride are added sequentially and stirred uniformly, the remaining sterile water is added, and the nursing disinfectant is obtained. The disinfectant is reasonable in compatibility, has the efficacy of sterilization, disinfection, heat clearing, damp drying, inflammation diminishing, itching relieving, tonifying, blood activating and the like, has remarkable curative effects, is safe to use and nonirritant, doesn't cause an allergic reaction, has relatively prominent nursing effects and can effectively prevent gynecological diseases.