The invention relates to the field of facility environment regulation and control, and discloses a positive-ventilation greenhouse environment regulation system, comprising an air-conditioning unit, blast tubes, a CO2 generator and an ozone generator, wherein air inlets of the blast tubes are connected with the air-conditioning unit, the blast tubes each is provided with a plurality of air outlets, the air-conditioning unit is used for filtering air, heating or cooling, humidifying or dehumidifying air, and air meeting requirements on greenhouse environment temperature and humidity is delivered to different position in the greenhouse in a positive-pressure state through the blast tubes. Besides, the CO2 generator is used for increasing concentration of CO2 in the greenhouse, and the ozonegenerator is used for generating ozone for disinfecting the greenhouse. According to the embodiment of the invention, the positive-ventilation greenhouse environment regulation system realizes comprehensive regulation and control on greenhouse environment in the positive-pressure greenhouse environment, delivers humid and cool air to the greenhouse space through the blast tubes uniformly, realizesquick air change and needs low construction cost.