The invention discloses a preparation method for a tree trunk whitening agent, and belongs to the technical field of agricultural material preparation. The preparation method for the tree trunk whitening agent comprises the following steps: first, sealing and fermenting bean curd, chilli powder, nutmeg powder and the like, filtering, collecting fermentation liquor, and mixing the fermentation liquor and sterile water, saccharose, citric acid and the like; then, performing constant temperature culture on a mixture, and mixing and fermenting with the plants, such as herba portulacae and chrysanthemum; sterilizing by utilizing ultraviolet light; collecting a fermented mixture, pulping, and performing centrifugal treatment with activated carbon; concentrating the obtained supernatant, and stirring uniformly with the supernatant, quick lime, hydroxymethyl cellulose and the like; finally, collecting the mixture, and performing sterilizing and disinfecting to obtain the tree trunk whitening agent. The tree trunk whitening agent prepared with the preparation method is purely white in colour and high in covering property; by adding the substances with high adhesive force and good film forming property, all of the adhesive property, the film forming property and the rainwater erosion resistance of the whitening agent can achieve a lasting effect; meanwhile, the whitening agent does not peel off easily after being used, has a relatively good heat preserving effect and a pest and disease damage preventing effect, is required to be applied only once, and has an excellent application prospect.