The invention discloses a light current microscopic device with
light modulation and real-time monitoring functions and a modulation method. The device comprises a bottom plate, a microscopic
system support, a sample table, an objective lens, a
microscopic imaging layer, a wide-field
light excitation layer and a point focusing
light excitation layer. Wherein the
microscopic imaging layer collimates, filters, reflects and focuses
broadband light to a rear focal plane of the objective lens to realize Kohler illumination, and sample light returned by the objective lens is reflected and imaged to the camera; the wide-field
light excitation layer collimates the wide-field light, adjusts the power, polarization direction and
light spot shape, and reflects and focuses the wide-field light to the rear focal plane of the objective lens to realize wide-field light excitation; after the point focusing light excitation layer collimates and reflects
laser and adjusts power, the polarization direction and the spot size are adjusted, and the
laser is focused to a sample table through an objective lens after being reflected for multiple times to achieve point focusing light excitation. The device can modulate the
laser wavelength, power and the like, monitors the
optical power in real time, meets the excitation
light modulation requirement, is compatible with more tests and reduces the operation complexity.