A tobacco seedling transplanting punching machine includes an engine, a throttle opening control cable, a clutch cable, transmission flexible shafts, punching drill bits, a vehicle frame, a water spray device, a power divider and a drill bit mounting rack; the vehicle frame includes a wheel, handlebars, a cross beam, landing legs, and a lifting handle, and a gradienter is arranged on the vehicle frame; the drill bit mounting rack includes cross rods, a fixing seat and connecting rods, tail ends of the two cross rods are symmetrically respectively arranged at two sides of the front end of the cross beam, the front end of each cross rod is connected with the connecting rod, the tail ends of the connecting rods are arranged on the fixing seat in a connection mode, and the punching drill bits are arranged below the fixing seat; power outputted by the engine is divided into two ways by the power divider to be outputted and then the two ways of power are connected with the transmission flexible shafts respectively, and the transmission flexible shafts pass through the fixing seat and then are connected with the punching drill bits respectively; the water spray device includes a water tank, a water spray pipe, a water pump and a water pump switch. One person can push the tobacco seedling transplanting punching machine for operation, so as to be convenient and save labor; with arrangement of two punching drill bits, two field ridges are punched at the same time, and the efficiency is high; and the water spay device ensures that holes cannot be collapsed.