The invention discloses a pumpkin jelly and a preparation method thereof. Raw materials for the pumpkin jelly comprise, by weight, 900 to 1000 parts of purified water, 20 to 40 parts of gelatin, 10 to 20 parts of agar, 40 to 55 parts of honey, 13 to 18 parts of cane sugar, 6 to 8 parts of green tea, 5 to 6 parts of mustard, 5 to 6 parts of heartleaf houttuynia herb, 5 to 6 parts of purslane herb, 3 to 5 parts of grape seeds, 3 to 5 parts of pumpkin seeds, 8 to 10 parts of carrot, 8 to 10 parts of brassica chinensis, 8 to 10 parts of apples, 10 to 12 parts of fried black soya bean, 10 to 12 parts of pumpkin, 1 to 2 parts of lycium barbarum polysaccharide and a proper amount of food coloring and potassium sorbate. According to the invention, gelatin and agar are mixed and used a gelatinizing agent, so pumpkin jelly not only contains collagen components but also has a softer and more delicious taste, is wet and slippery in mouth and dissolves as one chews, which enables the severe accident of choking because of swallowing to be completely prevented. The pumpkin jelly provided by the invention not only tastes like traditional jellies, but also can enhance health of human beings and promote growth of children and produces no side-effects when eaten for a long time.