The invention provides an engineered exosome for high expression of cancer suppression miRNA and targeting lung cancer, and also provides a preparation method of the engineered exosome, and application of the engineered exosome to drug loading and targeting therapy in lung cancer disease treatment. The engineered exosome for high expression of cancer suppression miRNA and targeting lung cancer can stably carry lung targeting alpha6beta1 protein, and wraps miRNAs: miR-214 and miR-770 for inhibiting growth and metastasis of lung cancer, so that the engineered exosome can be targeted to the lung and inhibit the lung cancer, and experimental results show that the produced engineered exosome has the characteristic of targeting lung cancer and can be used for preparing the lung cancer targeted exosome. Compared with the prior art, the engineering stable cell line has the advantages that the quantity of the exosome entering the lung tissue is increased, meanwhile, the produced engineering exosome carries high-peak miR-214 and miR-770, the concentration of miR-214 and miR-770 in the lung tissue is increased, the growth and metastasis of the lung cancer can be effectively inhibited, and in addition, the engineering stable cell line is capable of preserving breeds, convenient in passage and capable of continuously producing the engineering exosome.