The invention relates to a preparation process of antibiotic refreshing linear low density polyethylene film master batch, which uses LLDPE as carrier resin, adds with lanthanum oxide, zirconium phosphate loaded with silver, zirconium phosphate loaded with zinc, oleamide, silica dioxide, octadecanoic acid monoglyceride and oxidized polyethylene, and is prepared through dispersing by coating, modifying interface, mixing material and extruding and pilleting, wherein LLDPE is 40-49 parts by weight, lanthanum oxide is 10-15 parts by weight, zirconium phosphate loaded with silver is 8-12 parts by weight, zirconium phosphate loaded with zinc is 10-13 parts by weight, oleamide is 0.5-1.2-0 parts by weight, silica dioxide20-25 parts by weight, octadecanoic acid monoglyceride is 0.4-0.8 parts by weight and oxidized polyethylene is 1.2-1.8 parts by weight. Master batch which is prepared by the process is added in LLDPE resin according to the proportion of 4-5%, and the air permeable quantity isincreased 2-3 times compared with refreshing film with same thickness after prepared into film through film blowing molding, and the film is used to the refreshing package of fruits and vegetation, which can inhibit the generation of surface microbe, reduces fruit and vegetation from decaying, and prolongs refreshing time 15-45 days.